Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7802-7806.doi: 10.3969/j.issn.1673-8225.2010.42.004

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Immunological analysis of improved heterogeneous deproteinized bone for bone defects repair

Jian Yue-kui1, Luo Yang2, Tian Xiao-bin1, Li Bo1, Peng Zhi1, Ding Yi1, Luo Chun-shan1, Zhao Wei-feng1, Yang Zhen1   

  1. 1 Department of Orthopaedics, Guizhou Provincial People’s Hospital, Guiyang   550002, Guizhou Province, China; 2 Department of Laboratory Medicine, Southwest Hospital, the Third Military Medical University of Chinese PLA, Chongqing   400038, China
  • Online:2010-10-15 Published:2010-10-15
  • About author:Jian Yue-kui☆, Doctor, Associate chief physician, Department of Orthopaedics, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China jianyuekui@sina.com
  • Supported by:

    Guizhou Provincial Science and Technology Fund No.20082303*;200898*;20093019*

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Abstract:

BACKGROUND: The immunogenicity of heterogeneous bone remains unsolved in the choice of scaffold materials.
OBJECTIVE: To observe the immunological properties of heterogeneous deproteinized bone prepared by modified method.
METHODS: Eighteen goats of SPF grade were randomly divided into three groups, normal bone group without osteotomy, autologous bone group and heterogeneous deproteinized bone group were produced a 20% length of periosteum and bone defects in right tibial middle and inferior segments. Autologous bone group was implanted with autologous bone; in heterogeneous deproteinized bone group, goat autologous bone marrow mesenchymal stem cells were collected and adjusted into a density of 1×109/L, 2 mL cell suspension was incubated onto porcine deproteinized bone modified with porcine femoral bone, adding recombinant human bone morphogenetic protein 2, followed by semi-ring trough external fixation. The immunological detection of CD4+ and CD8+ T lymphocytes, serum antibodies IgG was performed using flow cytometry, bone defects repair was determined with dual-energy X-ray measurement.
RESULTS AND CONCLUSION: After heterogeneous deproteinized bone was implanted 3, 7, 14, 28 days, the levels of CD4+ and CD8+ T lymphocytes in peripheral blood were normal (P > 0.05), serum IgG antibody level was slightly higher than the autologous bone group (P > 0.05); at 24 weeks after implantation, bone mineral density and bone mineral content were similar to autologous bone group (P > 0.05), showed high-density calcification between the two ends of bone defect area. The pressure resistance and ultimate pressure, resistance to bending loads and limit load, torsion torque and ultimate torque were similar in three groups (P > 0.05). At 24 weeks after implantation, there was no significant difference in osteogenic potential between autologous bone group and heterogeneous deproteinized bone group (P > 0.05). The improved heterogeneous deproteinized bone does not cause significant cellular and humoral immune response, has good biocompatibility, does not affect the osteogenesis of autologous bone marrow mesenchymal stem cells, biomechanical properties of new bone are equal to autologous bone.

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