Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7797-7801.doi: 10.3969/j.issn.1673-8225.2010.42.003

Previous Articles     Next Articles

Repair of articular osteochondral defects with bone marrow stromal stem cells/nano-beta-tricalcium phosphate/collagen I and II composite

Chen Zhu-sheng1, Lü Yu-ming2, Zhang Bing1   

  1. 1 Department of Orthopaedics, The Second Traditional Chinese Medicine Hospital of Guangdong Province, Guangzhou   510095, Guangdong Province, China; 2 Department of Orthopaedics, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou   510150, Guangdong Province, China
  • Online:2010-10-15 Published:2010-10-15
  • Contact: Lü Yu-ming, Doctor, Associate chief physician, Department of Orthopaedics, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong Province, China lvyuming2008@126.com
  • About author:Chen Zhu-sheng☆, Doctor, Associate chief physician, Department of Orthopaedics, The Second Traditional Chinese Medicine Hospital of Guangdong Province, Guangzhou 510095, Guangdong Province, China czs8341@163.com
  • Supported by:

    Natural Science Foundation of Guangdong Province, No. 7002690*; Medical Scientific Research Fund of Guangdong Province, No. A2008128*; a grant by Guangzhou Medical College, No. 0709106*

Abstract:

BACKGROUND: Articular cartilage injury repair is mainly faced with the great difference in the structure and physiological functions between regenerated cartilage and normal cartilage, far away from normal physiological needs, there are many current repair methods, but the results are not satisfactory.
OBJECTIVE: To study the feasibility of repairing articular osteochondral defects in canines using nano-β-tricalcium phosphate (TCP)/collagen I and II composite loaded with bone marrow stromal stem cells (BMSCs).
METHODS: Ten 12-month-old hybrid canines were randomly divided into experimental group and defect control group, and BMSCs were sterilely obtained from iliac crest in each animal. Primary culture and subcultures were done. The third passage of BMSCs was digested and collected, cell density was adjusted to 2.0 × 106 L-1, the cells were co-cultured with nano- β-TCP/ colI/colII for 24 hours, then nano β-TCP/colI/colII/BMSCs composites were prepared. Articular osteochondral defects were made in canine right knees, those in experimental group were treated with nano β-TCP/colI/colII/BMSCs composites, while those in control group were left untreated.
RESULTS AND CONCLUSION: At 12 weeks, the defects in the experimental group were filled with white translucent tissues, which appeared smooth, soft and slightly protruded, chondrocytes distributed uniformly but aligned disorderly. At 24 weeks, the defects in the experimental group were filled with white translucent cartilage tissue, which appeared smooth and tenacious. The color and the luster were similar to that of the normal cartilage, and was ill-demarcated from the surrounding normal cartilage. The cells on the surface paralleled to joint surface. The cells in the deep layer arranged disorderly. In many areas, the cells clustered together. The matrix was extensively metachromatic and the new cartilage finely integrated with normal cartilage. In the control group, the defects showed little repair response macroscopically, only some white fibrous tissue can be seen at the bottom. BMSCs are ideal seed cells for repairing articular cartilage defects. Nano-β-TCP/colI/colII is gradually degraded and absorbed while new cartilage tissues form. It can be used as a suitable scaffold material for tissue engineered repair of articular cartilage defects.

CLC Number: