Antitumor activity and toxicity in vivo of iron-fluouracil complex

doi:10.3969/j.issn.2095-4344.2016.12.016

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (12): 1779-1786.doi: 10.3969/j.issn.2095-4344.2016.12.016

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Antitumor activity and toxicity in vivo of iron-fluouracil complex

Shi Min¹, Zhou Yun², Du Xin-e¹, Chen Ying-jie³, Wang Peng¹, Zhong Wen-yuan⁴, Zhou Yi-ping¹

¹School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, Yunnan Province, China; ²School of Chemistry, Yunnan Open University, Kunming 650223, Yunnan Province, China; ³Basic Medical College of Kunming Medical University, Kunming 650500, Yunnan Province, China; ⁴Department of Chemistry, Kunming College, Kunming 650000, Yunnan Province, China

Received:2016-02-01 Online:2016-03-18 Published:2016-03-18
Contact: Zhou Yi-ping, Associate professor, School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, Yunnan Province, China Corresponding author: Zhong Wen-yuan, Professor, Department of Chemistry, Kunming College, Kunming 650000, Yunnan Province, China
About author:Shi Min, Studying for master’s degree, School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, Yunnan Province, China
Supported by:
the Application Basic Research Joint Project of Yunnan Provincial Science and Technology Department & Kunming Medical University, No. 2014FB011; the Scientific Research Fund of Yunnan Provincial Education Department, No. 2014Z060

Abstract

Abstract:

BACKGROUND: Previous research indicated that iron-fluorouracil-phenanthroline complex has good antitumor activity in vitro, which can inhibit the proliferation of human cancer cells.
OBJECTIVE: To detect the antitumor activity and toxicity of iron-fluouracil-phenanthroline complex, [Fe(5-Fu)2(Phen)SO4], in vivo.
METHODS: A total of 40 Kunming mice were randomly divided into four groups, which were intraperitoneally injected with 72, 102.9, 147, 210 mg/kg [Fe(5-Fu)2(Phen)SO4] and the half lethal dose of the complex was detected. One day after the establishment of mouse S180 sarcoma models, the model mice were randomly divided into eight groups, and administered with the intraperitoneal injection of 15 mg/kg (low dose group), 30 mg/kg (middle dose group), 60 mg/kg (high dose group) [Fe(5-Fu)2(Phen)SO4], normal saline (negative control group), cisplatin (positive control group), 5-fluorouracil, iron-salt and phenanthroline, respectively. The injection was done once a day, lasting for 7 days. The weight of sarcomas, body weight, the main organ coefficient and histopathological changes of the main organs were detected.
RESULTS AND CONCLUSION: The half lethal dose of [Fe(5-Fu)2(Phen)SO4] was 103.9 mg/kg. Compared with the negative control group, high dose group, positive control group and 5-fluorouracil could significantly inhibit the growth of the tumor (P < 0.05 or P < 0.01), and the effect of high dose group was the most obvious (P < 0.01). Compared with cisplatin, 60mg/kg [Fe(5-Fu)2(Phen)SO4] had a weaker inhibitory effect on the kidney, but higher inhibitory effect on the liver, spleen and thymus, indicating the complex has a lower nephrotoxicity, but stronger immunotoxicity and hepatotoxicity than cisplatin.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Shi Min, Zhou Yun, Du Xin-e, Chen Ying-jie, Wang Peng, Zhong Wen-yuan, Zhou Yi-ping. Antitumor activity and toxicity in vivo of iron-fluouracil complex[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(12): 1779-1786.

Figures/Tables 5

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Antitumor activity and toxicity in vivo of iron-fluouracil complex

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