Effect of punicalagin on osteoclast activation induced by titanium particles

doi:10.3969/j.issn.2095-4344.2016.12.013

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (12): 1759-1765.doi: 10.3969/j.issn.2095-4344.2016.12.013

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Effect of punicalagin on osteoclast activation induced by titanium particles

Chu Geng-lei¹, Liu Si-han¹, Li Dong-ya², Li Hong-wei², Guo Kai-jin²

¹School of Graduate, Xuzhou Medical College, Xuzhou 221004, Jiangsu Province, China; ²Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China

Received:2016-02-09 Online:2016-03-18 Published:2016-03-18
Contact: Li Hong-wei, M.D., Chief physician, Master’s supervisor, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
About author:Chu Geng-lei, Studying for master’s degree, School of Graduate, Xuzhou Medical College, Xuzhou 221004, Jiangsu Province, China
Supported by:
the Surface Project of Health and Family Planning Commission of Jiangsu Province, No. H201528; the Project of XuZhou Science and Technology Department, No. KC14SH102

Abstract

Abstract:

BACKGROUND: Currently, there are few researches on the effect of punicalagin on the formation and differentiation of osteoclasts, and fewer researches on the mechanism of bone resorption diseases induced by wear particles.
OBJECTIVE: To establish a model of titanium particles induced mouse monocyte/macrophage cell line (RAW264.7) differentiating into osteoclasts and to observe the effect of different concentrations of punicalagins on osteoclast proliferation and differentiation.
METHODS: Mouse monocyte/macrophage cell lines (RAW264.7) were divided into five groups, cultured in the culture medium of common (blank group), 0.1 g/L titanium particle suspension, 0.1 g/L titanium particle suspension with 25 μmol/L punicalagins, 0.1 g/L titanium particle suspension with 50 μmol/L punicalagins, 0.1 g/L titanium particle suspension with 100 μmol/L punicalagins, respectively. The cell proliferative activity was detected by cell counting kit-8 assay at 1, 3 and 5 days. At 5 days after culture, number of osteoclasts was measured by tartrate-resistant acid phosphatase staining, the phosphorylation of IκBα and NF-κB p65 was detected by western blot assay, the mRNA expressions of nuclear factor of activated Tc1, tartrate-resistant acid phosphatase and matrix metalloproteinase-9 were measured by reverse transcription-PCR.
RESULTS AND CONCLUSION: Compared with control group, titanium particles and different concentrations of punicalagin had no effect on the proliferation of RAW264.7 cells (P > 0.05). The number of tartrate-resistant acid phosphatase staining -positive cells, the phosphorylation of IκBα and NF-κB p65 as well as the mRNA expressions of nuclear factor of activated Tc1, tartrate-resistant acid phosphatase and matrix metalloproteinase-9 were significantly increased compared with those of control group (P < 0.05，P < 0.01). And punicalagins in a concentration-dependent manner decreased the expression of the above indicators. These results indicate that punicalagin can inhibit osteoclast formation and differentiation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Chu Geng-lei, Liu Si-han, Li Dong-ya, Li Hong-wei, Guo Kai-jin. Effect of punicalagin on osteoclast activation induced by titanium particles[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(12): 1759-1765.

Figures/Tables 4

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Effect of punicalagin on osteoclast activation induced by titanium particles

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