Silk fibroin scaffold for repair of buccal mucosa defects

doi:10.3969/j.issn.2095-4344.2016.12.010

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (12): 1738-1744.doi: 10.3969/j.issn.2095-4344.2016.12.010

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Silk fibroin scaffold for repair of buccal mucosa defects

Wang Zhong-chao^{1, 2}, Fan Li-yuan^{1, 3}, Cai Wei^{1, 3}, Jiang Jun-qiang^{1, 2}

¹Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; ²Department of Periodontics & Oral Medicine, ³Department of Prosthodontics, Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China

Online:2016-03-18 Published:2016-03-18
Contact: Fan Li-yuan, Lecturer, Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Prosthodontics, Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
About author:Wang Zhong-chao, Master, Attending physician, Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Periodontics & Oral Medicine, Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
Supported by:
the Provincial-Level Innovation Training Plan for Undergraduates in the Stomatological School of Southwest Medical University, No. 201510632020; the University-Level Innovation Training Plan for Undergraduates in the Stomatological School of Southwest Medical University, No. 2015131; the Hospital-Level Project of the Hospital of Stomatology, Southwest Medical University; the National-Level Innovation Training Plan for Undergraduates in Southwest Medical University in 2015, No. 201510632020

Abstract

Abstract:

BACKGROUND: Fibroin is a natural macromolecular material with Arg-Gly-Asp peptide structure that is a special tripeptide structure closely related to cell adhesion, and it can promote cell migration, adhesion, and proliferation and influence cell morphology and function.
OBJECTIVE: To compare the effects of different silk fibroin scaffolds to repair buccal mucosa defects in rats.
METHODS: Ninety Sprague-Dawley rats were selected to make unilateral buccal mucosa defect models, and randomly divided into three groups, 30 rats in each group: porous silk fibroin scaffold was implanted into the buccal mucosa defect in experimental group, multi-layered crosslinked silk fibroin film was implanted into the buccal mucosa defect in control group, and vaseline gauze was used to cover the buccal mucosa defect followed by suturing in blank control group. After 15 days, wound diameter was detected; after 30 days, bone defect tissues were taken for hematoxylin-eosin staining.
RESULTS AND CONCLUSION: At postoperative 15 days, the wound diameter was significantly smaller in the experimental group than the control and blank control groups (P < 0.05), as well as smaller in the control group than the blank control group (P < 0.05). Hematoxylin-eosin staining showed that at 30 days after operation, there were more epithelial spikes and fibroblasts, but less inflammatory cells in the experimental group than the other two groups (P < 0.05), and fibroin fibers were partially absorbed and degraded in the experimental group. These findings indicate that porous silk fibroin scaffold for buccal mucosa defect repair can accelerate epithelialization and wound healing.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Wang Zhong-chao, Fan Li-yuan, Cai Wei, Jiang Jun-qiang. Silk fibroin scaffold for repair of buccal mucosa defects[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(12): 1738-1744.

Figures/Tables 3

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Silk fibroin scaffold for repair of buccal mucosa defects

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