Biocompatibility of poly-L-lactic acid/hydroxyapatite biomaterial with periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2016.12.009

Abstract

Abstract:

BACKGROUND: Hydroxyapatite/poly-L-lactic acid composite can well improve the brittleness and mechanical properties of the hydroxyapatite.
OBJECTIVE: To study the biocompatibility of hydroxyapatite/poly-L-lactic acid composite biomaterials with periodontal ligament cells.
METHODS: Passage 4 human periodontal ligament cells at a density of 5×109/L were cultured with hydroxyapatite/poly-L-lactic acid composite as experimental group, and human periodontal ligament cells cultured alone as control group. Cell proliferation was detected within 7 days of culture; alkaline phosphatase activity was detected at 24, 48 and 72 hours of culture; and type I collagen expression was detected at 72 hours of culture.
RESULTS AND CONCLUSION: There was no significant difference in the absorbance value of cells between the two groups at 1-7 days of culture. The alkaline phosphatase activity of cells at 48 and 72 hours was significantly higher than that at 24 hours (P < 0.05) in the two groups, but there was no difference between the two groups. The expression of type I collagen had no difference between the two groups. These findings indicate that the hydroxyapatite/poly-L-lactic acid composite biomaterial has good cell compatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Zhao Ning, Zhang Xian-jun, Li Wei, Dong Xin-xin . Biocompatibility of poly-L-lactic acid/hydroxyapatite biomaterial with periodontal ligament cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(12): 1732-1737.

Figures/Tables 5

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