Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (12): 1704-1710.doi: 10.3969/j.issn.2095-4344.2016.12.005

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Zinc-modified calcium silicate bioceramics coating and osteointegration

Xu Li-zhang1, Ye Xiao-jian1, Li Kai2, Zheng Xue-bin2, Tang Feng1, Xu PengXi1 , Xu Guo-hua1, Hou Chun-lin1, Yu Jiang-ming1   

  1. 1Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
  • Received:2016-01-11 Online:2016-03-18 Published:2016-03-18
  • Contact: Yu Jiang-ming, Attending physician, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • About author:Xu Li-zhang, Studying for master’s degree, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81301537; the National High Technology Research and Development Program of China (863 Program), No. 2013AA032203

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Abstract:

BACKGROUND: Zinc-modified calcium silicate (CaSiO3) bioceramics coating on the titanium surface prepared in preliminary experiments has good chemical stability and antibacterial property.
OBJECTIVE: To observe the effects of zinc-modified CaSiO3 bioceramics coating on osteointegration.
METHODS: MC3T3-E1 cells were respectively cultured on the titanium with zinc-modified CaSiO3 bioceramics coating (experiment group), titanium with CaSiO3 bioceramics coating (control group) and pure titanium (blank control group). Then, cell adhesion, proliferation, calcification rate and the expression of type I collagen and osteocalcin were detected. The implant materials mentioned above were respectively inserted into the femurs of New Zealand white rabbits, and after 1.5 months, the osteoproliferation and osteointegration between the implants and the host were tested.
RESULTS AND CONCLUSION: In vitro experiment: The number of adhesive cells at 12 hours after co-culture was significantly increased in the experimental group compared with the control group and blank control group (P < 0.05). At 14 days after co-culture, cell proliferation ability and ability of calcium nodule formation in the experiment group were significantly better than those in the other groups (P < 0.05). At 21 days after co-culture, there was no significant difference in the expression of type I collagen, but the expression of osteocalcin in the experiment group was higher than that in the control group and blank control group (P < 0.05). In vivo experiment: In the experiment group, a large amount of bone substances were detected, the coating materials directly contacted with the bone interface, new bone tissues and little fibrous tissues were observed at the interface. In contrast, there was a small amount of bone hyperplasia in the control group and almost no bone hyperplase in the blank control group. Moreover, a small part of the implant directly contacted with the bone interface and the most part was separated from bone trabeculae by fibrous tissues. These findings indicate that zinc-modified CaSiO3 bioceramics coating can enhance the ability of osteointegration between titanium implants and the host.