Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (11): 1591-1596.doi: 10.3969/j.issn.2095-4344.2016.11.011

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Dexamethasone inhibits the expression of microRNA-155 in macrophages induced by lipopolysaccharide

Wang Zhong-hua, Wang Shou-hong, Wu Yan, Li Zhou, Liao Xiao-long, Qin Tie-he   

  1. Guangdong Provincial Institute of Geriatrics, Guangdong General Hospital (Guangdong Academy of Medical Science), Guangzhou 510080, Guangdong Province, China
  • Received:2016-02-10 Online:2016-03-11 Published:2016-03-11
  • Contact: Qin Tie-he, Chief physician, Guangdong Provincial Institute of Geriatrics, Guangdong General Hospital (Guangdong Academy of Medical Science), Guangzhou 510080, Guangdong Province, China
  • About author:Wang Zhong-hua, M.D., Guangdong Provincial Institute of Geriatrics, Guangdong General Hospital (Guangdong Academy of Medical Science), Guangzhou 510080, Guangdong Province, China

Abstract:

BACKGROUND: It is unclear about dexamethasone effect on the regulation of microRNA-155 expression in macrophages.
OBJCTIVE: To explore whether dexamethasone can regulate the expression of microRNA-155 in macrophages.
METHODS: (1) Lipopolysaccharide stimulation of mouse macrophages: mouse macrophage cell lines, Raw264.7 cells, were cultured in vitro and stimulated by lipopolysaccharide. Cultured cells were collected at 0, 0.5, 2, 6 hours after culture to detect the dynamical expression of microRNA-155. (2) Dexamethasone intervention for macrophages: Macrophages were divided into four groups: control group treated with phosphate buffer; lipopolysaccharide group stimulated by lipopolysaccharide; combined group given intervention with dexamethasone and lipopolysaccharide; dexamethasone group cultured with dexamethasone. At 6 hours after culture, cell supernatant was collected to detect the expression of tumor necrosis factor α and interleukin-6 using ELISA method. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-155 in the Raw264.7 macrophages.
RESULTS AND CONCLUSION: Lipopolysaccharide significantly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 after 6 hours of culture (P < 0.05). Combined use of dexamethasone and lipopolysaccharide slightly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 (P < 0.05). Dexamethasone alone had no influence on the expression of tumor necrosis factor α and interleukin-6, but significantly decreased the expression of microRNA-155 (P < 0.05). These findings indicate that dexamethasone can inhibit the expression of microRNA-155 in the macrophages induced by lipopolysaccharide.