Effect of gastric cancer stem cells CSC-G on invasion and metastasis of gastric cancer cells

doi:10.3969/j.issn.2095-4344.2016.11.012

Abstract

Abstract:

BACKGROUND: Studies have shown that cancer stem cells play an important role in tumor invasion and metastasis, but studies on the role of gastric cancer stem cells in invasion and metastasis of gastric cancer cells were rarely reported.
OBJECTIVE: To study the effect of gastric cancer stem cells CSC-G on invasion and metastasis of gastric cancer cells.
METHODS: Gastric cancer stem cells CSC-G and gastric cancer cells SGC7901 were cultured in vitro for 10 days followed by spherical colony formation assay, western blot assay for detecting OCT4, SOX2, E-cadherin and CD44 protein expression levels in gastric cancer stem cells CSC-G and gastric cancer cells SGC7901, and Transwell assay for detecting the invasion and migration of gastric cancer stem cells and gastric cancer cells SGC7901.
RESULTS AND CONCLUSION: Gastric cancer cells SGC7901 in RPMI1640 medium presented with adherent growth and were quadrilateral or polygonal after passage; gastric cancer stem cells CSC-G in serum-free medium presented with suspension growth, and adherent gastric cancer stem cells were spindle-shaped or round. Compared with gastric cancer cells SGC7901, the protein expressions of OCT4, SOX2 and CD44, the number of cancer cell spheres and the number of trans-membrane cells were significantly increased in the gastric cancer stem cells CSC-G (P < 0.05), and the expression of E-cadherin protein was significantly decreased (P < 0.05). These findings indicate that the gastric cancer stem cells CSC-G can be successfully cultured in vitro, and have enhanced invasion and migration compared with the gastric cancer cells SGC7901, which play an important role in gastric cancer invasion and metastasis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Li Xiang-hui, Wang Gui-ji. Effect of gastric cancer stem cells CSC-G on invasion and metastasis of gastric cancer cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(11): 1597-1602.

Figures/Tables 3

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