Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (8): 1118-1125.doi: 10.3969/j.issn.2095-4344.2016.08.008

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In vitro release and antibacterial property of minocycline hydroxyapatite/chitosan complex

Gao Hao-chen1, Wang Pei1, Cao Zhi-zhong1, Ge Kui-kui2, Wang Yi-han3, Lu Min3   

  1. 1Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China; 3Shanghai High-Tech United Bio-Technological R&D Co., Ltd., Shanghai 201206, China
  • Received:2016-01-04 Online:2016-02-19 Published:2016-02-19
  • Contact: Cao Zhi-zhong, M.D., Chief physician, Professor, Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • About author:Gao Hao-chen, Studying for master’s degree, Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Supported by:

    the Scientific Research Project of Shanghai Science and Technology Committee, No. 411951202; the Postgraduate Innovative Experimental Project of the Second Military Medical University, No. SCMRC1405

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Abstract:

BACKGROUND: Hydroxyapatite/chitosan (HA/CS) complex may act as a drug carrier for drug release, but little is reported about the release amount and antibacterial effect of minocycline-HA/CS (Mino-HA/CS) complex.
OBJECTIVE: To investigate the in vitro release and antibacterial property of Mino-HA/CS complex.
METHODS: HA/CS and Mino-HA/CS were prepared using co-precipitation method. The surface and cross-section features of the complexes were observed under scanning electron microscopy. The porosities were measured according to Archimedes Principle. The release of minocycline hydrochloride was measured by high performance liquid chromatography with the simulated saliva as drug release media. In vitro antibacterial effect on Porphyromonas gingivalis and Staphylococcus aureus were measured by bacteria-inhibiting ring method. Biological toxicities were evaluated via cell counting kit-8cell proliferation assay.
RESULTS AND CONCLUSION: The porosity of Mino-HA/CS was larger than that of HA/CS, with the average porosity of 53.99%. Single-day release amount of Mino-HA/CS could maintain at the level of 0.5-1 μg per day for a long-term. Bacteriostatic rings of Porphyromonas gingivalis and Staphylococcus aureus still existed clearly after 7 days. Cell proliferation assays showed that Mino-HA/CS extract had the significant effect on promoting cell proliferation. These findings indicate that the Mino-HA/CS sustains the release of minocycline at a relatively stable level within a longer period, shows good inhibitory effect on Porphyromonas gingivalis and Staphylococcus aureus and promotes the proliferation of periodontal ligament cells.