Effect of basic fibroblast growth factor and insulin-like growth factor-1 on proliferation and collagen synthesis of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2016.06.019

Abstract

Abstract:

BACKGROUND: How to control the orderly formation of collage in skin repair and scarring process is worthy of attention.
OBJECTIVE: To investigate the effect of basic fibroblast growth factor (bFGF) combined with insulin-like growth factor 1 (IGF-1) on the proliferation and collagen synthesis of rat bone marrow mesenchymal stem cells in vitro.
METHODS: Rat bone marrow mesenchymal stem cells were isolated and cultured to induce adipogenic differentiation assessed by oil red O staining and osteogenic differentiation identified by alizarin red staining in vitro. Passage 3 cells were cultured in the medium containing bFGF, IGF-1, combination of them or the control fluid, respectively. MTT assay was used to detect cell proliferation at 12, 24, 48, 72 and 96 hours of culture. The expression of type I collagen and type III collagen were detected by RT-PCR and western blot after 10 days of incubation.
RESULTS AND CONCLUSION: Compared with the control group, bFGF or IGF-1 alone significantly promoted the proliferation of bone marrow mesenchymal stem cells, and inhibited the expression of type I collagen and type III collagen. After combined use of bFGF and IGF-1, the proliferation of bone marrow mesenchymal stem cells was improved more significantly, and the expression of type I collagen and type III collagen returned to normal levels. These findings indicate that the combination of IGF-1 and bFGF can promote proliferation of bone marrow mesenchymal stem cells and restrain the expression of type I collagen and type III collagen, which may be helpful for control and repair of scar formation during wound healing.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Xu Hai-long, Ding Yue, Xie Hong, Sun Xiao-ju, Xie Hui-xin. Effect of basic fibroblast growth factor and insulin-like growth factor-1 on proliferation and collagen synthesis of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(6): 891-897.

Figures/Tables 4

References

[1] Leoni G, Neumann PA, Sumagin R, et al. Wound repair: role of immune-epithelial interactions. Mucosal Immunol. 2015;8(5):959-968.

[2] Hagemann JH, Haegele H, Müller S, et al. Danger control programs cause tissue injury and remodeling. Int J Mol Sci. 2013;14(6):11319-11346.

[3] Hurtley S, Hines PJ, Mueller KL, et al. Skin. From bench to bedside. Introduction.Science. 2014;346 (6212):932-933.

[4] Fabi S, Sundaram H. The potential of topical and injectable growth factors and cytokines for skin rejuvenation. Facial Plast Surg. 2014;30(2):157-171.

[5] Wang H, Chen Z, Li XJ, et al. Anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in a rabbit ear model. Eur J Pharmacol. 2015;751:42-49.

[6] Parekkadan B, Milwid JM. Mesenchymal stem cells as therapeutics. Annu Rev Biomed Eng. 2010;12:87-117.

[7] Crane JL, Cao X. Bone marrow mesenchymal stem cells and TGF-β signaling in bone remodeling. J Clin Invest. 2014;124(2):466-472.

[8] Alhadlaq A, Mao JJ. Mesenchymal stem cells: isolation and therapeutics Stem Cells Dev. 2004;13(4):436-448.

[9] Augello A, Kurth TB, De Bari C. Mesenchymal stem cells: a perspective from in vitro cultures to in vivo migration and niches. Eur Cell Mater. 2010;20:121- 133.

[10] Huang S, Wu Y, Gao D, et al. Paracrine action of mesenchymal stromal cells delivered by microspheres contributes to cutaneous wound healing and prevents scar formation in mice. Cytotherapy. 2015;17(7): 922-931.

[11] Duffield JS, Lupher M, Thannickal VJ, et al. Host responses in tissue repair and fibrosis. Annu Rev Pathol. 2013;8:241-276.

[12] Buchanan EP, Longaker MT, Lorenz HP. Fetal skin wound healing. Adv Clin Chem. 2009;48:137-161.

[13] Lee DE, Ayoub N, Agrawal DK. Mesenchymal stem cells and cutaneous wound healing: novel methods to increase cell delivery and therapeutic efficacy. Stem Cell Res Ther. 2016;7(1):37.

[14] Du L, Lv R, Yang X, et al. Hypoxic conditioned medium of placenta-derived mesenchymal stem cells protects against scar formation.Life Sci. 2016;149:51-57.

[15] Doi H, Kitajima Y, Luo L, et al. Potency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing. Sci Rep. 2016; 6:18844.

[16] Tong C, Hao H, Xia L, et al. Hypoxia pretreatment of bone marrow-derived mesenchymal stem cells seeded in a collagen-chitosan sponge scaffold promotes skin wound healing in diabetic rats with hindlimb ischemia. Wound Repair Regen. 2015 Oct 14. [Epub ahead of print]

[17] Abd-Allah SH, El-Shal AS, Shalaby SM, et al. The role of placenta-derived mesenchymal stem cells in healing of induced full-thickness skin wound in a mouse model. IUBMB Life. 2015;67(9):701-709.

[18] Oh SY, Lee SJ, Jung YH, et al. Arachidonic acid promotes skin wound healing through induction of human MSC migration by MT3-MMP-mediated fibronectin degradation. Cell Death Dis. 2015;6: e1750.

[19] Caruana G, Bertozzi N, Boschi E, et al. Role of adipose-derived stem cells in chronic cutaneous wound healing.Ann Ital Chir. 2015;86(1):1-4.

[20] Chen D, Hao H, Fu X, et al. Insight into Reepithelialization: How Do Mesenchymal Stem Cells Perform. Stem Cells Int. 2016;2016:6120173.

[21] Basiouny HS, Salama NM, Maadawi ZM, et al. Effect of bone marrow derived mesenchymal stem cells on healing of induced full-thickness skin wounds in albino rat. Int J Stem Cells. 2013;6(1):12-25.

[22] Tan CQ, Gao X, Guo L, et al. Exogenous IL-4-expressing bone marrow mesenchymal stem cells for the treatment of autoimmune sensorineural hearing loss in a guinea pig model. Biomed Res Int. 2014;2014: 856019.

[23] Snyder TN, Madhavan K, Intrator M, et al. A fibrin/hyaluronic acid hydrogel for the delivery of mesenchymal stem cells and potential for articular cartilage repair. J Biol Eng. 2014;8:10.

[24] Kankuri E, Mervaala EE, Storvik M, et al. Exacerbation of acute kidney injury by bone marrow stromal cells from rats with persistent renin-angiotensin system activation. Clin Sci (Lond). 2015;128(11):735-747.

[25] Delcroix GJ, Garbayo E, Sindji L, et al. The therapeutic potential of human multipotent mesenchymal stromal cells combined with pharmacologically active microcarriers transplanted in hemi-parkinsonian rats. Biomaterials. 2011;32(6):1560-1573.

[26] Amarnath S, Foley JE, Farthing DE, et al. Bone marrow-derived mesenchymal stromal cells harness purinergenic signaling to tolerize human Th1 cells in vivo. Stem Cells. 2015;33(4):1200-1212.

[27] Huang C, Orbay H, Tobita M, et al. Proapoptotic effect of control-released basic fibroblast growth factor on skin wound healing in a diabetic mouse model. Wound Repair Regen. 2015 Oct 21. [Epub ahead of print]

[28] Kondo T, Ishida Y. Molecular pathology of wound healing. Forensic Sci Int. 2010;203(1-3):93-98.

[29] Kondo T.Timing of skin wounds. Leg Med (Tokyo). 2007;9(2):109-114.

[30] Moulin V. Growth factors in skin wound healing. Eur J Cell Biol. 1995;68(1):1-7.

[31] Xuan YH, Huang BB, Tian HS, et al. High-glucose inhibits human fibroblast cell migration in wound healing via repression of bFGF-regulating JNK phosphorylation. PLoS One. 2014;9(9):e108182.

[32] Liao MH, Liu SS, Peng IC, et al. The stimulatory effects of alpha1-adrenergic receptors on TGF-beta1, IGF-1 and hyaluronan production in human skin fibroblasts. Cell Tissue Res. 2014;357(3):681-693.

[33] Jacków J, Schlosser A, Sormunen R, et al. Generation of a Functional Non-Shedding Collagen XVII Mouse Model: Relevance of Collagen XVII Shedding in Wound Healing. J Invest Dermatol. 2016;136(2): 516-525.

[34] Theocharidis G, Drymoussi Z, Kao AP, et al. Type VI Collagen Regulates Dermal Matrix Assembly and Fibroblast Motility. J Invest Dermatol. 2016;136(1): 74-83.

[35] Shi H, Cheng Y, Ye J, et al. bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1- JNK Pathways. Int J Biol Sci. 2015;11(7):845-859.

[36] Nagayoshi M, Taguchi T, Koyama H, et al. Enhanced neovascular formation in a novel hydrogel matrix consisting of citric Acid and collagen. Ann Vasc Dis. 2011;4(3):196-203.

[37] Wang JJ, Liu YL, Sun YC, et al. Basic Fibroblast Growth Factor Stimulates the Proliferation of Bone Marrow Mesenchymal Stem Cells in Giant Panda (Ailuropoda melanoleuca). PLoS One. 2015;10(9): e0137712.

[38] Colenci R, da Silva Assunção LR, Mogami Bomfim SR, et al. Bone marrow mesenchymal stem cells stimulated by bFGF up-regulated protein expression in comparison with periodontal fibroblasts in vitro. Arch Oral Biol. 2014;59(3):268-276.

[39] Kinoshita T, Ishikawa Y, Arita M, et al. Antifibrotic response of cardiac fibroblasts in hypertensive hearts through enhanced TIMP-1 expression by basic fibroblast growth factor. Cardiovasc Pathol. 2014; 23(2):92-100.

[40] Shi HX, Lin C, Lin BB, et al. The anti-scar effects of basic fibroblast growth factor on the wound repair in vitro and in vivo. PLoS One. 2013;8(4):e59966.