Biological properties of human periodontal ligament stem cells under inflammatory microenvironment

doi:10.3969/j.issn.2095-4344.2016.06.020

Abstract

Abstract:

BACKGROUND: The periodontal ligament stem cells can promote periodontal tissue regeneration, providing a new way for the treatment of periodontitis.

OBJECTIVE: To observe the inflammatory microenvironment effects on the biological properties of periodontal ligament stem cells.

METHODS: Periodontal ligament stem cells from healthy controls and patients with periodontitis were primarily cultured by tissue digestion method, purified using limited dilution method, and identified through detection of CD146 and STRO-1. Then, passage 3 cells were taken and denoted as normal control and inflammation groups followed by osteogenic induction.

RESULTS AND CONCLUSION: Purified cells from two sources both expressed STRO-1 and CD146. Periodontal ligament stem cells in the inflammation group showed higher multiplication capacity, but the osteogenesis ability was lower compared with the normal control group. The expressions of Runx2 mRNA and Osterix mRNA were dropped significantly after the stimulus of tumor necrosis factor-α

(P < 0.05), but the interleukin-1β and interleukin-6 did not have a significant impact. Tumor necrosis factor-α at 0.1 and 1 μg/L had no significant effects on the expression of Runx2 mRNA, but the expression of Runx2 mRNA was decreased significantly after treatment with 10 μg/L tumor necrosis factor-α (P < 0.05). It is confirmed that the molecular signaling mechanism inside the periodontal ligament stem cells is changed under inflammatory microenvironment, so that the differentiation capacity of cells from the inflammatory sources is lowered. Moreover, tumor necrosis factor-α is one of the key factors and its optimal concentration is 10 μg/L.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Yuan Ping, Li Shu-hui, Zhao Lu, Yu Li, Zhou Chun-mei, Wu Pei-ling . Biological properties of human periodontal ligament stem cells under inflammatory microenvironment[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(6): 898-905.

Figures/Tables 6

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