Proliferation and differentiation of mesenchymal stem cells from the synovial tissue in patients with osteoarthritis

doi:10.3969/j.issn.2095-4344.2015.41.001

Abstract

Abstract:

BACKGROUND: With respect to mesenchymal stem cells from other sources, synovial mesenchymal stem cells are rich in source, and moreover, the synovial tissue can regenerate quickly after partial hepatectomy and lead to fewer complications, in recent year, which have become a hot spot in stem cell research.
OBJECTIVE: To observe the proliferation and directional differentiation of synovial mesenchymal stem cells from osteoarthritis patients.
METHODS: Synovial mesenchymal stem cells were isolated and cultured. MTT assay was used to detect cell proliferation ability. Alkaline phosphatase activity was detected quantitatively at 7 days of osteogenic induction, and osteogensis-related gene expression was measured at 7, 14, 21 days of osteogenic induction. Alizarin red staining was performed at 21 days of induction.
RESULTS AND CONCLUSION: (1) Passage 3 synovial mesenchymal stem cells proliferated faster, which were in latent period at 1, 2 days after inoculation, in logarithmic growth phase at 3-6 days, and then entered into the plateau phase at 7 days. (2) The activity of alkaline phosphatase was significantly higher in the induction group than the control group at 3, 7, 10 days after osteogenic induction (P < 0.05). The cells were positive for alizarin red staining at 21 days of osteogenic induction, and there were calcium deposits and calcium nodules in the extracellular matrix. (3) Bone-binding protein and Runx2 were visible at 7 days of osteogenic induction, and reached the peak at 21 days. These findings indicate that synovial mesenchymal stem cells from patients with advanced osteoarthritis have strong proliferation ability, which can differentiate into osteoblasts under in vitro induction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Knee, Synovial Membrane, Mesenchymal Stem Cells, Cell Proliferation, Cell Differentiation, Tissue Engineering

CLC Number:

R394.2

Chen Lu, Xia Xian-xue, Jiang Ke. Proliferation and differentiation of mesenchymal stem cells from the synovial tissue in patients with osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2015.41.001.

Figures/Tables 5

References

[1] 张平平,向川.骨髓间充质干细胞治疗骨关节炎：可能与未来[J].中国组织工程研究,2014,18(6):968-973.
[2] 郑洁,王瑞辉,寇久社.炎性反应在骨关节炎软骨退变中的作用[J].基础医学与临床,2014,34(8):1146-1149.
[3] De Bari C, Dell'Accio F, Tylzanowski P, et al. Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum. 2001;44(8):1928-1942.
[4] 芮云峰,林禹丞,陈辉,等.晚期骨关节炎患者膝关节滑膜间充质干细胞的体外成骨分化[J].中国组织工程研究,2013,17(45): 7840-7846.
[5] 杨勇,Tien Huey-y,陈山林,等.韧带重建肌腱团填塞术治疗第一腕掌关节骨关节炎的疗效分析[J].中华骨科杂志,2014,34(10): 1030-1036.
[6] 高文香,郝军.筋病理论指导下中医综合疗法治疗膝骨关节炎[J].中医正骨,2014,26(1):60-62.
[7] 孙奎,杨骏,沈德凯,等.隔附子饼灸治疗肝肾不足型膝原发性骨关节炎[J].中国针灸,2008,28(2):87-90.
[8] 徐宁,刘蕊,臧嘉捷,等.草乌甲素片治疗骨关节炎效果和安全性评价[J].中国医药,2014,9(8):1170-1173.
[9] 汪利合.玻璃酸钠关节腔注射与运动疗法治疗髋臼发育不良性髋关节骨关节炎[J].中国组织工程研究与临床康复,2010,14(29): 5403-5406.
[10] 黎国权,覃海宁.关节置换治疗老年膝关节退行性骨关节炎的疗效[J].中国老年学杂志,2013,33(22):5543-5545.
[11] 郭洪亮,帖小佳,韩亚军,等.定向诱导骨关节炎患者骨髓间充质干细胞向软骨细胞的分化[J].中国组织工程研究,2015,19(6): 832-836.
[12] 甘凤英,唐琛,郭迪斌,等.间充质干细胞移植治疗膝关节骨关节炎临床观察[J].现代诊断与治疗,2014,25(15):3512-3513.
[13] 李治,赵伟,刘伟,等.玻璃酸钠及胎盘间充质干细胞和诱导的软骨细胞膝关节腔内注射：修复膝骨关节炎[J].中国组织工程研究, 2014,18(50):8140-8146.
[14] Campbell WG Jr, Callahan BC. Regeneration of synovium of rabbit knees after total chemical synovectomy by ingrowth of connective tissue-forming elements from adjacent bone. A light and electron microscopic study. Lab Invest. 1971;24(5): 404-422.
[15] Bentley G, Kreutner A, Ferguson AB. Synovial regeneration and articular cartilage changes after synovectomy in normal and steroid-treated rabbits. J Bone Joint Surg Br. 1975;57(4): 454-462.
[16] Owen TA, Aronow M, Shalhoub V, et al. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol. 1990;143(3):420-430.
[17] 徐凌霄,王芳,郭敦明,等.左归丸对间充质干细胞向软骨细胞分化过程中Ⅱ型胶原及蛋白多糖基因表达的影响[J].中国中西医结合杂志,2011,31(12):1662-1668.
[18] 李海东.血清性激素结合蛋白和RANK/RANKL/OPG系统在绝经后女性骨关节炎和骨质疏松中的作用研究[D].上海:上海交通大学,2011.
[19] 牟方政,李荣亨,张文亮,等. 复元胶囊对兔膝骨关节炎软骨中胰岛素样生长因子-I及胰岛素样生长因子结合蛋白3表达的影响[J].中国老年学杂志,2011,31(12):2229-2231.
[20] 王伟卓,程一钊,郭雄,等.大骨节病差异表达基因PAPSS2对成骨细胞矿化及碱性磷酸酶活性的影响[J].西安交通大学学报:医学版,2014,35(2):175-181.