Establishing mouse models of allergic rhinitis by knocking out H2-eb1 gene

doi:10.3969/j.issn.2095-4344.2015.27.028

Abstract

Abstract:

BACKGROUND: HLA-DRB1 is related to the pathogenesis of allergic rhinitis. Construction of HLA-DRB1 gene knockout animal models not only elucidates the pathogenesis of allergic rhinitis, but also provides a good way for the elucidation of the pathogenesis of allergic rhinitis-related diseases.
OBJECTIVE: To establish the HLA-DRB1 gene knockout animal models.
METHODS: Homozygous, wild-type and heterozygous mice were obtained by inbreeding of the heterozygous mice. Confirmed by gene and protein identification, 24 female wild-type (H2-eb1+/+) mice and 12 H2-eb1-/- mice aged 8 weeks were selected according to the random number table. 12 H2-eb1+/+ mice and 12 H2-eb1-/- mice were sensitized with ovalbumin to establish the mouse models of allergic rhinitis. Another 12 mice were sensitized with PBS as comparison.
RESULTS AND CONCLUSION: Compared with the control mice, serum levels of ovalbumin-specific IgE and interleukin-4 were significantly increased, while serum level of γ-interferon was significantly decreased in the mouse models of allergic rhinitis. Serum levels of IgE and interleukin-4 were lower, while serumγ- interferon level was higher, in H2-eb1-/- gene knockout mice of allergic rhinitis than those in the H2-eb1+/+ gene knockout wild-type mice. These results suggest that H2-eb1 gene may play an important role in regulating Th1/Th2 imbalance in the pathogenesis of allergic rhinitis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Rhinitis, Allergic, Perennial, Gene Knockout Techniques, Inbreeding, Th1/Th2 Balance

CLC Number:

R318

Li Lin-ge, Feng Juan, Hu Bin, Shou Xi, Zhang Chun, Tian Yu, Jiang Chun-rong, Zhang Yu, Zhang Hua . Establishing mouse models of allergic rhinitis by knocking out H2-eb1 gene [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(27): 4417-4422.

Figures/Tables 5

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