Identification and differentiation of breast cancer stem cells under tumor microenvironment

doi:10.3969/j.issn.2095-4344.2015.14.005

Abstract

Abstract:

BACKGROUND: Breast cancer stem cells have a greater impact on the occurrence and metastasis of breast cancer. Under simulated tumor microenvironment, we can better analyze the proliferation and differentiation of breast cancer stem cells.

OBJECTIVE: To explore the tumor microenvironment effect on the differentiation of breast cancer stem cells.

METHODS: Breast cancer cells and MCF-7 cells were primarily cultured in fibroblast supernatant and serum-free PCM-2 medium, and formation of breast cancer cells microspheres was observed. Proliferative ability of breast cancer cells was detected using MTT colorimetry, and the surface markers of breast cancer stem cells and epithelial-mesenchymal transition markers were measured using immunocytochemistry and RT-PCR methods.

RESULTS AND CONCLUSION: The diameter of primary cell microspheres was larger in the serum-free PCM-2 medium than in the fibroblast supernatant, but the culture speed was faster in the fibroblast supernatant than the serum-free PCM-2 medium. At 3 days of primary culture, the expression of ALDH1 in primary cells was greatly higher in the serum-free PCM-2 medium than in the fibroblast supernatant. However, the expressions of E-cadherin and vimentin were up-regulated in the fibroblast supernatant than in the serum-free PCM-2 medium. In addition, the expressions of E-cadherin and vimentin in MCF-7 cells cultured in the fibroblast supernatant were up-regulated, while the expressions of ALDH1 and Oct-4 were downregulated. These findings indicate that the tumor environment has some certain effects on the growth and differentiation of breast cancer stem cells, and some cytokines secreted from fibroblast supernatant can promote the proliferation and differentiation of breast cancer stem cell microspheres to some extent.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Breast Neoplasms, Tumor Microenvironment, Microspheres

CLC Number:

R394.2

Zhang Shu-qing, Zhang Bo, Hong Liang, Zhou Yong-an, Zhao Liang. Identification and differentiation of breast cancer stem cells under tumor microenvironment[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(14): 2155-2160.

Figures/Tables 2

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