Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (5): 794-798.doi: 10.3969/j.issn.2095-4344.2015.05.024

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Cross-reactivity of tree shrew sera with various secondary antibodies: extensive application to tree shrew models of diseases

Ruan Guang-ping, Yao Xiang, Liu Ju-fen, Wang Jin-xiang, He Jie, Yang Jian-yong, Pan Xing-hua   

  1. Stem Cell Engineering Laboratory, Kunming General Hospital of Chengdu Military Area Command of Chinese PLA, Kunming 650032, Yunnan Province, China
  • Revised:2014-11-12 Online:2015-01-30 Published:2015-03-02
  • Contact: Pan Xing-hua, M.D., Chief physician, Stem Cell Engineering Laboratory, Kunming General Hospital of Chengdu Military Area Command of Chinese PLA, Kunming 650032, Yunnan Province, China
  • About author:Ruan Guang-ping, M.D., Associate chief physician, Stem Cell Engineering Laboratory, Kunming General Hospital of Chengdu Military Area Command of Chinese PLA, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31172170; the National Key Basic Research and Development Program (973 Program), No. 2012CB518106; the Special Project of High-New Technology Industrial Development in Yunnan Province, No. 201204; the National Support Program of China, No. 2014BAI01B01

Abstract:

BACKGROUND: Tree shrew is a representative between insectivore and primates, has a high degree of evolution, is more inexpensive primates, has high use of medical biology, and has been attached by scholars.
OBJECTIVE: To detect whether the commonly used secondary antibodies have immune response with tree shrew serum.
METHODS: Western blot assay and enzyme linked immunosorbent assay were utilized to detect whether the tree shrew serum had cross-reacts with anti-rabbit, anti-goat, anti-human, anti-mouse, anti-rat, and anti-monkey secondary antibodies.
RESULTS AND CONCLUSION: Western blot assay results indicated that tree shrew serums did not react with anti-rabbit, anti-goat, anti-human, anti-mouse, and anti-rat secondary antibodies and had cross reaction with anti-monkey secondary antibody. Enzyme linked immunosorbent assay results also indicated that tree shrew serums were cross-reactive with anti-monkey secondary antibody, but did not have cross-reactivity with the other secondary antibodies. Above data confirmed that the usually soled secondary antibody cannot be used to immunoassay with tree shrews IgG. Only anti-monkey secondary antibody has cross-react with tree shrew serum. It is necessary to prepare anti-tree shrew IgG monoclonal and polyclonal antibodies. When no antibody is readily available at present, anti-monkey secondary antibody can be used to substitute detection, and can be widely applied in the study of tree shrew models of disease.



中国组织工程研究
杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程


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Key words: Tupaiidae, Receptors, IgG, Cross Reactions

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