Stromal cell derived factor 1 effects on migration of endogenous neural stem cells

doi:10.3969/j.issn.2095-4344.2014.06.021

Abstract

Abstract:

BACKGROUND:Stromal cell derived factor 1 in chemotactic migration of endogenous neural stem cells plays a very important role, but the specific migration mechanism is unclear

OBJECTIVE: To observe the effects of exogenous stromal cell derived factor 1 on chemotactic migration and proliferation of neural stem cells in the rat hippocampus

METHODS:Exogenous stromal cell derived factor 1 (5 μL, 500 μ/L) was injected into the hippocampus of Sprague-Dawley rats to establish animal models. Brain tissues were taken after days 3, 7, 14 and 21 of perfusion to prepare paraffin sections. Thereafter, nestin expression in the injection region and hippocampus was detected using immunohistochemical method. Experimental control and blank control groups were set.

RESULTS AND CONCLUSION: Paraffin section immunohistochemical results displayed the number of nestin-positive cells in the injection and the hippocampus was gradually increased. At 3 and 7 days, nestin expression was a little and increased at 14 days, forming a migration tendency to the injection region. At 21 days, there were more nestin-positive cells in the injection area and hippocampus. However, there were no changes as above in the experimental control and blank control groups. The results showed that exogenous stromal cell derived factor 1 may induce the proliferation of neural stem cells in the hippocampus and may be involved in chemotactic migration of endogenous neural stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: neural stem cells, chemokine CXCL12, hippocampus, cell movement

CLC Number:

R394.2

Su Wen, Ding Peng, Wang Jin-kun, Zhang Hao, Mu Lin-jie, Wang Bo, Liu Jing-chuan, Gong Guang-hui, Wang Chong-qian. Stromal cell derived factor 1 effects on migration of endogenous neural stem cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2014.06.021.

Figures/Tables 3

2.1 实验动物数量分析 68只雄性SD大鼠参加实验，中途如有未到时间窗时间死亡的，则按相同数量补充。 2.2 SD大鼠海马大脑皮质内注射外源性基质细胞衍生因子1模型的神经行为表现大鼠皮质内注射外源性基质细胞衍生因子1后大部分无特别表现，极少部分出现烦躁不安、毛发耸立约10 min后自行缓解，课题组分析可能与对麻药的个体差异有关。 2.3 苏木精-伊红染色光镜观察结果空白对照组大鼠脑组织苏木精-伊红染色示脑组织无明显病变。实验组及实验对照组各时间窗注射区皮质染色示：大部分未见异常，极少部分见轻微水肿，在所有实验组与实验对照组的切片中均未发现皮质注射区出血及感染灶，证实了实验建模成功(图1)。 2.4 皮质注射区nestin蛋白免疫组化染色及定量检测结果 Nestin蛋白免疫反应阳性产物为棕黄色点状或颗粒状沉积，主要在细胞质内，有的可见到核分裂。空白对照组大鼠皮质区未见nestin 阳性细胞表达。实验组造模3 d见少量nestin阳性细胞在注射皮质区表达。造模7 d注射皮质区表达nestin阳性细胞稍增多。造模14 d见nestin阳性细胞排列成线状向注射区聚集，尖端指向注射区。建模21 d注射区周围大量nestin 阳性细胞。实验对照组各时间窗注射皮质区nestin阳性细胞有少量表达，但未发现随时间窗推移逐渐增多趋势(图2)。空白对照组nestin蛋白在皮质区未见表达，实验对照组在皮质注射区见有nestin 蛋白阳性细胞表达，但表达较少，各时间点nestin蛋白累积吸光度值比较，差异无显著性意义(P > 0.05)。实验组nestin蛋白累积吸光度值在各个时间点均高于同时间的实验对照组、空白对照组，差异有显著性意义(P < 0.01)。实验各组之间的两两比较差异有显著性意义(P < 0.01，表1)。"

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