Effect of different concentrations of uric acid on the neural differentiation of human bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.06.005

Abstract

Abstract:

BACKGROUND: Uric acid as an endogenous antioxidant has garnered increasing attentions because of its anti-oxidation, anti-DNA damage and neuroprotective effects.

OBJECTIVE: To observe the effect of uric acid at different concentrations on the neural differentiation of bone marrow mesenchymal stem cells.

METHODS: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro. The morphology change was observed. The third passages of bone marrow mesenchymal stem cells were induced to differentiate to neuron-like cells by induced liquid containing four different concentrations of uric acid

(0 mmol/L as control group, 0.2 mmol/L, 0.4 mmol/L, 0.8 mmol/L) for 24 hours. Then, after second intervention for 1 hour, cells were detected by Nissl staining and specific markers were detected by immunohistochemistry method.

RESULTS AND CONCLUSION: After induction, the cell body shrank, forming processes and connections. Nissl body was found in the cytoplasm. The positive rates of neuron-specific enolase were significantly higher in uric acid groups of different concentrations compared to the control group (P < 0.05); moreover, the positive rates of neuron-specific enolase were increased as the increase in concentrations of uric acid (P < 0.05). The positive rates of Nestin were decreased in uric acid groups of different concentrations compared to the control group(P < 0.05). After 4 hours of induction, cells fell off significantly. In a certain period of time, uric acid can promote differentiation of bone marrow mesenchymal stem cells into neuron-like cells in a certain concentration-dependent manner in vitro.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, bone marrow, cell differentiation, neurons, cell adhesion molecules, neuronal

CLC Number:

R394.2

Song Qing-qing, Yu Ling-fan, Xu Li-li, Yang Nai-long. Effect of different concentrations of uric acid on the neural differentiation of human bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(6): 847-852.

Figures/Tables 5

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