Biosafety of prednisolone implantable film

doi:10.3969/j.issn.2095-4344.2013.51.011

Abstract

Abstract:

BACKGROUND: After peripheral nerve injury, to inhibit scar formation by drugs is the key to functional recovery. To reduce the amount of scar formation we designed a prednisolone-loaded film which can sustain drug release and good achievement in in vitro drug release test.
OBJECTIVE: To prepare the prednisolone implantable film and investigate its in vivo biocompatibility and safety.
METHODS: Prednisolone-loaded nanoparticles were first prepared with reverse micellar emulsion-solvent evaporation method, and the composite film and drug-loaded film were further prepared. Then, we investigated the in vivo biocompatibility of drug-loaded film through cell toxicity test, hemolysis test, acute systemic toxicity test, chronic systemic toxicity test.
RESULTS AND CONCLUSION: After cultured for 7 days, the relative growth rate of L929 mouse fibroblasts was 92.6%, showing no cytotoxicity. The hemolysis rate of the film was 0.59%, indicating that the material had no hemolysis action. No abnormal biological behaviors were seen in mice after intraperitoneal injection of film extracts, and there were no changes in liver and renal functions in rats. As illustrated above, we can safely come to a conclusion that prednisolone-loaded film possesses good biocompatibility and can be safely used in the experiment of reducing the scar at sites of peripheral nerve repair.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: toxicity tests, histocompatibility, injections, intraperitoneal, cicatrix

CLC Number:

R318

Tang Yu-sen, Li Qiang, Qi Peng, Lian Ke-jian. Biosafety of prednisolone implantable film[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8849-8855.

Figures/Tables 5

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