Some leukocyte differentiation antigens express in side population cells in leukemia K562 cell line

doi:10.3969/j.issn.2095-4344.2013.49.021

Abstract

Abstract:

BACKGROUND: To study the phenotypes of side population cells in leukemia is important for understanding the heterogeneity and origin of tumor cells, molecular markers and targeted therapy.
OBJECTIVE: To identify whether the human chronic myeloid leukemia cell line-K562 contains side population cells or not, and to further observe the differences in expressions of leukocyte differentiation antigens from side population cell subset and non-side population cells subset.
METHODS: Flow cytometry was used to detect whether there were side population cells in the K562 cell lines. Then, the expression of CD34⁺, CD34⁺CD38^-, CD34⁺CD38⁺, HLA-DR⁺ cells in the side population subsets and non-side population subsets.
RESULTS AND CONCLUSION: Flow cytometry results showed that the K562 cell line contained side population cells, and the proportion of side population cells was much lower. The side population cells accounted for (2.7±0.5)% of viable cells in K562. The expressions of CD34⁺ cells and CD34⁺CD38^- cells in the side population subset were significantly higher than those in the non-side population subsets. The expressions of CD34⁺CD38⁺ cells and HLA-DR⁺ cells in the side population subset and non-side population subset did not have a significant difference. Heterogeneity was found in the differentiation antigen expression between the side population subset and non-side population subset.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, leukemia, myelogenous, chronic, BCR-ABL positive, K562 cells, flow cytometry, antigens, differentiation

CLC Number:

R394.2

Fan Rui-hua, Guo Dian-xuan, Zhang Tie-cheng, Yue Shun, Huang Ming-de, Yao Rong . Some leukocyte differentiation antigens express in side population cells in leukemia K562 cell line[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8596-8601.

Figures/Tables 2

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