Abstract:

BACKGROUND: Both carboxymethyl chitosan and platelet-derived growth factor-BB can promote the proliferation of human periodontal ligament cells cultured in vitro.
OBJECTIVE: To explore the effects of carboxymethyl chitosan and platelet-derived growth factor-BB combination on the proliferation and differentiation of human periodontal ligament cells cultured in vitro.
METHODS: The fourth and the fifth generation of human periodontal ligament cells were collected and primarily cultured in different groups: control group (Dulbecco’s modified Eagle’s medium contained 2% fetal bovine serum), 10 μg/L platelet-derived growth factor-BB group, 100 mg/L carboxymethyl chitosan+10 μg/L platelet-derived growth factor-BB group, 800 mg/L carboxymethyl chitosan group+10 μg/L platelet-derived growth factor-BB group, 100 mg/L carboxymethyl chitosan group and 800 mg/L carboxymethyl chitosan group.
RESULTS AND CONCLUSION: (1) Compared with control group, each experimental group can promote the proliferation ability of human periodontal ligament cells by MTT assay, and the proliferation of the cells in the combination groups was higher than that in the other groups (P < 0.05), and the dose of 100 mg/L carboxymethyl chitosan plus 10 μg/L platelet-derived growth factor-BB had the greatest promotion effect on the proliferation of human periodontal ligament cells (P < 0.05). (2) The cell cycle results were in line with the results of the MTT assay. (3) Alkaline phosphatase activity detection: compared with the control group, the alkaline phosphatase activity was increased in all groups except for 10 μg/L platelet-derived growth factor-BB group (P < 0.05). It indicates that the combination of carboxymethyl chitosan and platelet-derived growth factor-BB can promote the proliferation and osteogenic differentiation of the human periodontal ligament cells.