Abstract:

BACKGROUND: Bacterial cellulose is a new type of biological polymer materials, characterized by the advantages such as three-dimensional structure and high hydrophilicity.
OBJECTIVE: To investigate the biocompatibility of the natural polymer bacterial cellulose by co-cultured with cells in vitro.
METHODS: Bacterial cellulose scaffold was prepared with static culture program. Then the C2C12 cells and L929 cells in exponential phase of growth were co-cultured with bacterial cellulose in vitro. The morphology of bacterial cellulose scaffold was observed with scanning electron microscope. The growth and the proliferation of the cells were observed by using phase contrast microscope and the cell proliferation rate was measured by Cell Counting Kit-8 colorimetry.
RESULTS AND CONCLUSION: Bacterial cellulose had a fine three-dimensional structure and the fiber diameter was in the nano-level. The C2C12 cells and L929 cells could in vitro grew and proliferate on the bacterial cellulose scaffold and showed fusiform shape; and their morphology did not changed significantly. The relative proliferation rates of C2C12 cells and L929 cells on the bacterial cellulose scaffold were ≥100%. The cytotoxicity grade of bacterial cellulose was 0. Bacterial cellulose has good biocompatibility which is benefit to cell adhesion and proliferation.