Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (49): 9152-9156.doi: 10.3969/j.issn.2095-4344.2012.49.006

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Migration of bone marrow mesenchymal stem cells and changes in infarct volume in rats with cerebral ischemia under mild hypothermia 

Zhang Qi-mei1, Peng Yu1, You Hui1, Li Shou-hua2, Yan Guo-shan3, Zhang Zhao-wen1, Yang Bo1, Lan Jing1, Zheng Zheng1, Chi Ying1   

  1. 1Department of Neurology, the First Clinical Medical College, Yichang Central People’s Hospital, Three Gorges University, Yichang 443003, Hubei Province, China; 2Department of Neurology, Yichang Yiling Hospital, Yichang 443100, Hubei Province, China; 3Department of Neurology, Dangyang People’s Hospital, Dangyang 444100, Hubei Province, China
  • Received:2012-05-07 Revised:2012-07-16 Online:2012-12-02 Published:2013-01-16
  • Contact: Peng Yu, Master, Associate chief physician, Department of Neurology, the First Clinical Medical College, Yichang Central People’s Hospital, Three Gorges University, Yichang 443003, Hubei Province, China ycpengu@126.com
  • About author:Zhang Qi-mei★, Master, Chief physician, Department of Neurology, the First Clinical Medical College, Yichang Central People’s Hospital, Three Gorges University, Yichang 443003, Hubei Province, China Ycpengyu123@126.com
  • Supported by:

    Research and Development Projects of Hubei Science and Technology Bureau*; a grant from the First Clinical Medical College of Three Gorges University, No. A11301-05*

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Abstract:

BACKGROUND: There are a few reports addressing the application of mild hypothermia to the study of repairing the nerve injury, but few reports have addressed the effects of mild hypothermia on the migration of neural stem cells in the brain following transplantation.
OBJECTIVE: To explore the effects of mild hypothermia on the migration of bone marrow mesenchymal stem cells transplanted into the lateral ventricle of rats with cerebral ischemia, as well as the effect on the infarct volume.
METHODS: A focal cerebral ischemic injury model of right middle cerebral artery occlusion was established using modified Longa’s method in Sprague-Dawley rats. Fifty rats were divided into mild hypothermia group, control group and sham-operation group. Local mild hypothermia was applied in the mild hypothermia group before transplantation for the treatment of acute cerebral ischemia. Normal body temperature was maintained in control group before transplantation for the treatment of acute cerebral ischemia. The right carotid artery of rats in the sham-operation group was separated and ligated after anesthesia. At 24 hours following model establishment in the mild hypothermia group and the control group, bone marrow mesenchymal stem cells labeled with 5-BrdU were transplanted into the rat lateral ventricle. The amount of BrdU-positive cells in the brain tissue in each group was measured by immunohistochemistry at 5, 14 and 21 days following injection in each group.  
RESULTS AND CONCLUSION: At 14 days after transplantation, a majority of labeled bone marrow mesenchymal stem cells had migrated to the infarct area. At various time points following transplantation, the number of BrdU-positive cells in the cortex around infarction focus was obviously greater in the hypothermia group compared with the control group  (P < 0.05). Compared with the control group, the infarct volume in the mild hypothermia group at various time points was reduced significantly (P < 0.05). Results indicate that mild hypothermia before transplantation may promote the direct migration of bone marrow mesenchymal stem cells, and can reduce the infarct volume.

CLC Number: 

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R394.2