Abstract:

BACKGROUND: The antibacterial activity of phenytoin in wound healing process is still not clear.
OBJECTIVE: To observe the antibacterial activity of phenytoin in the wound healing process through in vitro and animal experiment.
METHODS: ①In vitro experiment: Casein hydrolyzate broth dilution was used to observe the minimum inhibitory concentration, and agar diffusion method was used to observe in vitro antibacterial activity of 1.024 mg/L phenytoin. ②Animal experiments: Thirty Sprague Dawley rats were divided into three equal groups; a 2 cmx3 cm rectangular wound was created in rat dorsal paraspinal, and then treated with 10 or 20 mg/cm² phenytoin paste or pure Vaseline gauze, the wound was bandaged and fixed with Vaseline yarn and dry gauze. The dressing was changed every day, and the wound surface bacteria were quantitative cultured at 4, 8, 12 and 16 days.
RESULTS AND CONCLUSION: ①Casein broth dilution method results: The quality control strains and clinical isolates test were performed, the test tubes of different phenytoin concentration showed a turbid state. ②Agar diffusion method results: The quality control strains test was performed, and no inhibition zone formation was observed. ③Experimental animal wound bacteria quantitative culture results: The bacteria quantization in the group treated with 10 or 20 mg/cm² phenytoin paste was lower than that in the group treated with pure Vaseline gauze, but there was no significant difference between groups (P > 0.05). The results show that the in vitro or in vivo antibacterial activity of phenytoin is not clear, and it has no significant effect on the wound bacterial clearance.