Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (42): 7914-7918.doi: 10.3969/j.issn.2095-4344.2012.42.024

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Changes of the atrophic parotid gland induced by duct ligation

Zhao Teng-da1, Zuo Jin-hua1, Yang Yong1, Wang Li-fang1, Song Shou-jun1, Ding Chang-ling1, Zhu Yu-hong2, Gao Xu1   

  1. 1Department of Oral and Maxillofacial Surgery, 2Department of Pathology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China
  • Received:2011-12-03 Revised:2012-01-30 Online:2012-10-14 Published:2012-10-14
  • Contact: Zuo Jin-hua, Chief physician, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China jinhua256603@163.com
  • About author:Zhao Teng-da★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China zhaotengdakq@163.com

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Abstract:

BACKGROUND: The human major salivary glands are usually affected by various factors, such as radiation therapy, Sjogren's syndrome and sialadenitis, which can lead to atrophy. At present, there are only few studies on the morphological changes of long-term atrophic parotid gland.
OBJECTIVE: To investigate the changes of the atrophic parotid gland induced by its main duct ligation.
METHODS: After the duct ligation of the atrophic parotid gland induced by its right main duct of SD rats, the area of inner acinar and duct cells of normal parotid gland and atrophic parotid gland tissues were examined by hematoxylin-eosin staining after duct ligation for 0 (control), 1, 3, 7, 14, 30 and 60 days. Changes in number and distribution of myoepithelial cells in the atrophic parotid gland at different time points were analyzed by immunohistochemical staining method.
RESULTS AND CONCLUSION: Morphological analysis showed that rapid apoptosis in the acinar cells appeared after the ligation of the main duct in the parotid gland, and cells almost disappeared at day 14. With the atrophy of the parotid gland, a gradual decrease and fibrosis of the glandular lobules with inflammatory cell infiltration appeared. And a number of duct-like structures formed. Besides, the area of duct was gradually increased, and then was gradually decreased after reaching the peak at day 14. Duct-like structures showed a classic double-layers structure. The areas of acinar and duct in the experimental group were significantly different from those in the control group (P < 0.05). At day 7 after ligation, quantitative analysis showed significant increase in the number of myoepithelial cells, followed by gradual increases, which, however, were not statistically significant. These results suggest that in the earlier atrophic stage, there was a rapid disappearance of acinar accompanied by the occurrence of duct-like structures, and reactive myoepithelial cells proliferation. Ultimately, the “double-layers” structures composed by myoepithelial cells and duct-like structures may have great significances in the process of resisting atrophy.

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