Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (42): 7909-7913.doi: 10.3969/j.issn.2095-4344.2012.42.023

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Effect of cortical electrical stimulation on neurofilament protein 200 expression in ischemic stroke rats

Cheng Ling-yan1, Li Tao1, Zhou Hai-han2, Cheng Xuan1, Zhang Qian1, Tan Jie1, Li Cheng-yan1, Duan Yan-wen2   

  1. 1First Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
    2College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, China
  • Received:2011-12-29 Revised:2012-02-14 Online:2012-10-14 Published:2012-10-14
  • Contact: Li Tao, Doctor, Associate professor, Master’s supervisor, First Department of neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China ltlll@163.com
  • About author:Cheng Ling-yan★, Studying for master's degree, First Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China lingyan_0618 @163.com

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Abstract:

BACKGROUND: Early studies have shown that cortical electrical stimulation (CES) alone for 16 days can enhance dyskinesia and the expression of microtubule-associated protein 2 (MAP2) in peri-infarct cortex after cerebral ischemia in rats.
OBJECTIVE: To investigate the effects of CES on the expression of neurofilament protein 200 (NF-200) in rats model of ischemic stroke.
METHODS: Sprague-Dawley rats model of right middle cerebral artery occlusion in ischemic stroke were established. The rats with lesions in the cortex were randomly divided into CES group (n=13) and non-stimulation group (n=10). One week after modeled, electrical stimulators were implanted and the electrodes were placed on the surface of peri-infarct cortex. CES group was given the electric stimulation, but not in the non-stimulation group. CES was terminated after implantation for 14 days. And then the expression of NF-200 in the peri-infarct and contralateral cortex was assessed by immunofluorescence image analysis at 6 weeks after CES started.
RESULTS AND CONCLUSION: In the peri-infarct and contralateral cortex, the expression of NF-200 in the CES group was higher than that in the non-stimulation group (P < 0.05). These results suggest that CES can increase the expression of NF-200 in both peri-infarct and contralateral cortex, and induce the plasticity of bilateral cortical neuron axons.

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