Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (42): 7883-7887.doi: 10.3969/j.issn.2095-4344.2012.42.018

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Preparation and evaluation of contact lens electrode in mice

Zheng Qin-xiang1, Zhu Jia-li1, Pan Miao-zhen1, Shi Ying1, 2, Li Wen-sheng1, 3   

  1. 1Optometry & Ophthalmology Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China
    2RGP Research and Development Centre, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China
    3Department of Ophthalmology, University of Massachusetts Medical School, Worcester 01545, MA, USA
  • Received:2012-02-08 Revised:2012-03-19 Online:2012-10-14 Published:2012-10-14
  • Contact: Li Wen-sheng, Professor, Doctoral supervisor, Optometry & Ophthalmology Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China; Department of Ophthalmology, University of Massachusetts Medical School, Worcester 01545, MA, USA Worcester, MA, USA drlws@163.net
  • About author:Zheng Qin-xiang☆, Doctor, Lecturer, Optometry & Ophthalmology Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China zhengqinxiang@yahoo.com.cn

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Abstract:

BACKGROUND: Mice are the animal model used most commonly for studying the occurrence, development and prevention of human retinal diseases. Retinal electrophysiological tests have become one of the routine methods for the test of mouse retina function.
OBJECTIVE: To develop a new kind of contact lens electrode for mouse corneal electroretinogram (ERG) recordings, and to detect its effectiveness and repeatability in the process of the test.
METHODS: First, the cornea radius of curvature and the cornea diameter of 24 C57BL6/J mice at various ages were measured. Contact lens in mice of different ages were prepared according to the measured biological parameters of mouse cornea and processed by lathe digital controlled system. The electrode was then adhered to the contact lens and became a new kind of ERG electrode. Another 12 C57BL6/J mice aged 6 weeks were averagely divided into two groups and performed ERG test with traditional annular metallic electrode and this new designed contact lens electrode, respectively. B-wave amplitudes of both dark-adapted and light-adapted ERG in the two groups were recorded every two weeks, totally three times. Cornea status of mice was evaluated by slit lamp microscope after 2 weeks.
RESULTS AND CONCLUSION: There was significant difference in cornea diameter of different age mice (1.39- 1.48 mm). The designed radius of curvature of the cornea was 2.00 mm and corneal diameter was 3.5 mm. The mean values of B-wave amplitudes of both dark-adapted and light-adapted ERG using the contact lens electrode were recorded 82.7% and 80.3% of those tested by the traditional annular electrode. However the stability of contact lens electrode was much higher than that of the traditional annular electrode after repeated testing. ERG test by using contact lens electrode showed that mouse corneal neovascularization in the contact lens electrode group was obviously less than that in the traditional electrode group. These results suggest that mouse contact lens electrode can be efficiently applied in ERG test. Although its b-wave amplitude was lower than that of traditional annular electrode, its stability in repeated test was higher, and it could effectively decrease mouse corneal injuries.

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