Abstract:

BACKGROUND: At present, there are several methods for preparing a model of severe acute pancreatitis (SAP) in mice, including Hyla method with multiple injection, L-arginine-induced method with high dose and choline deficiency food feeding. But these methods all have their limiations, and therefore, they cannot be used widely.
OBJECTIVE: To explore and establish a new type model of SAP in mice.
METHODS: Forty adult male ICR mice were collected randomly and divided into two groups (each of 20 mice): sham operation group and model group. The mice of the sham operation group were only performed laparotomy and closure. The mice of the model group were injected retrogradely into biliopancreatic duct with 3% sodium taurocholate under macroscopic observation. All mice were killed under anesthesia at hours 6, 12, 24 and 48 after injection. Serum amylase, alanine aminotransferase, creatinine and lactate dehydrogenase were assayed. Histological scores of pancreas and lung of the two groups were examined under microscope.
RESULTS AND CONCLUSION: Activities of serum amylase, serum alanine aminotransferase, serum creatinine and serum lactate dehydrogenase of the model group were significantly higher than those of the sham operation group at each time point (P < 0.05). Histopathological scores: interstitial edema, inflammatory cells (mononuclear and neutrophil cells) infiltration, necrosis as well as parenchymal hemorrahages in the model group were higher than those in the sham operation group (P < 0.05), and lesions of other organs including the lung, liver and kidney were found. These results suggest that compared with other methods, the mice model of SAP induced by sodium taurocholate under macroscopic observation is easy, reproducible and stable, and is in accordance with clinical diseases.