Abstract:

BACKGROUND: Cementoblasts play an important role during root formation, cementum regeneration of periodontitis and repair of orthodontic root resorption. The influence of mechanical stimulation on cementoblasts in vitro is rarely reported.
OBJECTIVE: To analyze the relationship between different stress patterns at tension and compression sides during orthodontic tooth movement and cementum remodeling.
METHODS: 2000 με tensile and compressive stress was exerted, separately, on cementoblast-like cells OCCM30 by four-point bending system. The flow cytometry was used to examine the cell cycle and proliferation activity of OCCM30 after 3-, 6-, 12-, 24-hour loading.
RESULTS AND CONCLUSION: 2000 με tensile and compressive stress both depressed proliferation activity of OCCM30 through decreasing S-phase fraction after 3 and 6 hours loading. The S-phase fraction rose again with time prolonged. After 24 hours loading, the S-phase fraction and proliferation activity resumed to the level of controls. There was no significant difference between tensile and compressive stress in the influence on proliferation activity.