Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (42): 7814-7818.doi: 10.3969/j.issn.2095-4344.2012.42.005

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Expressions of cyclooxygenase 2, bone morphogenetic protein 2 and vascular endothelial growth factor in elbow heterotopic ossification

Tian Jian1, Fan Cun-yi2, Rui Yong-jun1, Mi Jing-yi1, Ruan Hong-jiang2, Liu Kun2, Zeng Bing-fang2   

  1. 1Department of Hand Surgery, the 9th People’s Hospital of Wuxi, Wuxi 214061, Jiangsu Province, China
    2Deprtment of Orthopedics, the 6th People’s Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 200233, China
  • Received:2012-01-13 Revised:2012-03-11 Online:2012-10-14 Published:2012-10-14
  • Contact: Fan Cun-yi, Doctor, Chief physician, Department of Orthopedics, the 6th People’s Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 200233, China fancunyi888@hotmail.com
  • About author:Tian Jian★, Master, Attending physician, Department of Hand Surgery, the 9th People’s Hospital of Wuxi, Wuxi 214061, Jiangsu Province, China tianjian828@163.com

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Abstract:

BACKGROUND: Several studies have shown that cyclooxygenase 2 (COX-2) inhibitors can prevent heterotopic ossification around the elbow joint.
OBJECTIVE: To investigate the expression of COX-2, bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF) in post-traumatic elbow heterotopic ossification tissues, and to analyze their relationship.
METHODS: The expressions of COX-2, BMP-2 and VEGF were examined by S-P immunohistochemical staining in 18 cases of heterotopic ossification and 10 cases of normal bone tissue. The 10 cases were as controls. The average absorbance value and the percentage of positive area of COX-2, BMP-2 and VEGF in heterotopic ossification and normal bone tissue were measured by HPIAS-1000 image analysis system. The relationship among the percentage of the positive area in the three kinds of proteins was analyzed.
RESULTS AND CONCLUSION: COX-2 ,BMP-2 and VEGF were strongly expressed in heterotopic ossification，but those in the normal bone tissue showed low express or no express. Image analysis demonstrated that the average absorbance value and the percentage of positive area of COX-2, BMP-2 and VEGF in heterotopic ossification were significantly higher than those in the normal bone tissue (P < 0.01). The percentage of positive area of COX-2 expression was closely correlated with that in the BMP-2 and VEGF in heterotopic ossification (P < 0.01). These findings suggest that COX-2, BMP-2 and VEGF may play important roles in the process of heterotopic ossification formation. COX-2 may induce the expression of BMP-2 and VEGF to promote osteogenesis and angiogenesis in the heterotopic ossification organization.

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