Abstract:

BACKGROUND: Nano hydroxyapatite/polycaprolactone with excellent biocompatibility and biological activity is a typical biological compound material.
OBJECTIVE: To investigate the feasibility of nano hydroxyapatite/polycaprolactone electrospun film as scaffold materials for bone tissue engineering
METHODS: First, nano hydroxyapatite/polycaprolactone electrospun film was prepared by electrostatic spinning technology. Second, the nano hydroxyapatite/polycaprolactone electrospun film was co-cultured in vitro with passage 3 bone marrow mesenchymal stem cells of rats. Then, bone marrow mesenchymal stem cells were induced into osteoblasts by dexamethasone, β-glycerophophate and vitamin C.
RESULTS AND CONCLUSION: Nano hydroxyapatite/polycaprolactone scaffolds possessed good macro porous structure, and pores were interconnected with each other. ①Inverted microscope observation: After bone marrow mesenchymal stem cells and the scaffold were co-cultured for 7 days, cells were mostly fusiform and began to proliferate. After 14 days, cell growth was more vigorous, and the number of cells was increased significantly. Moreover, cells secreted the extracellular matrix and adhered to the scaffold. ②Scanning electron microscope observation: At day 7, a large number of cells grew in scaffold pores, and they had active proliferation capacity. Besides, cells were mostly fusiform, and had good bipolar protrusions. In addition, the cells showed three- dimensional-like growth and secreted the extracellular matrix, as well as fibronectin was produced. These results suggest that the nano hydroxyapatite/polycaprolactone scaffold has good biocompatibility, and it can be used as a good carrier for bone tissue engineering.