Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (37): 6942-6946.doi: 10.3969/j.issn.2095-4344.2012.37.021

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Effects of over-expressed integrin-linked kinase on rat neonatal cardiomyocyte proliferation

Wang Bing-jian1, Xu Biao2, Bai Jian2, Li Qian-jun1, Sun Jing-xian3, Zhang Na2   

  1. 1Huaian First Hospital of Nanjing Medical University, Huaian 223300, Jiangsu Province, China
    2Department of Cardiology, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
    3Department of Cardiology, Jiangsu Province Official Hospital, Nanjing 210029, Jiangsu Province, China
  • Received:2012-05-09 Revised:2012-06-18 Online:2012-09-09 Published:2012-09-09
  • About author:Wang Bing-jian★, Master, Attending physician, Huaian First Hospital of Nanjing Medical University, Huaian 223300, Jiangsu Province, China wangbingjian@medmail.com.cn

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Abstract:

BACKGROUND: Integrin-linked kinase plays an important role in regulating cell survival and anti-apoptosis to promote cell migration, proliferation, and differentiation.
OBJECTIVE: To explore the effects of over-expressed integrin-linked kinase on cardiomyocytes proliferation in neonatal rats.
METHODS: The heart of neonatal rats (1–3 days old) was extracted to in vitro isolate and culture cardiomyocytes for 72 hours. Then, the cells were transfected with recombinant adenovirus vector or recombinant adenovirus vector+ integrin-linked kinase gene.
RESULTS AND CONCLUSION: Compared with the recombinant adenovirus vector group, there was an increase in DNA synthesis (P < 0.05), mitosis (P < 0.05) and the number of cardiomyocytes (P < 0.05) in the recombinant adenovirus vector+ integrin-linked kinase gene group. Briefly, integrin-linked kinase can promote the proliferation of neonatal rat cardiomyocytes.

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