Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (37): 6871-6875.doi: 10.3969/j.issn.2095-4344.2012.37.007

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Construction of an animal model of intervertebral disc degeneration and expression of bone morphogenetic protein-7

Li Gang-jian1, Zhao Xin2   

  1. 1Department of Orthopedics, Guangming Hospital of Traditional Chinese Medicine, Shanghai 201300, China
    2Department of Orthopedics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201360, China
  • Received:2012-05-12 Revised:2012-06-21 Online:2012-09-09 Published:2012-09-09
  • About author:Li Gang-jian, Attending physician, Department of Orthopedics, Guangming Hospital of Traditional Chinese Medicine, Shanghai 201300, China ligangjian666@hotmail.com

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Abstract:

BACKGROUND: Bone morphogenetic protein-7 can promote extracellular matrix synthesis in the intervertebral disc tissue.
OBJECTIVE: To observe the expression of bone morphogenetic protein-7 in rabbits with intervertebral disc degeneration.
METHODS: Models of intervertebral disc degeneration were established in New Zealand white rabbits using nucleus aspiration. The lumbar intervertebral disc of L3/4, L4/5, L5/6 were exposed in control group without treatment. The changes in the intervertebral disc were observed by MRI after modeling. The contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen were measured in 12 weeks later.
RESULTS AND CONCLUSION: Intervertebral disc degeneration occurred at 2 weeks after modeling detected by using MRI. Compared with the control group, the disc height, T2 value, contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen in the model group were significantly decreased at 12 weeks after modeling. It indicated that the expression of bone morphogenetic protein-7 was declined in the degenerative intervertebral disc of rabbits induced by nucleus aspiration method.

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