Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (36): 6758-6761.doi: 10.3969/j.issn.2095-4344.2012.36.019

Previous Articles     Next Articles

The active fractions of eucommia bark for osteogenic induction of bone marrow mesenchymal stem cells

Zhu Li-hua1, Zhang Xian1, Zhang Yan-hong2, Tan Xiang-ling2   

  1. 1Wuxi Hospital of Traditional Chinese Medicine, Wuxi 214001, Jiangsu Province, China;
    2College of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China
  • Received:2011-12-11 Revised:2012-01-17 Online:2012-09-02 Published:2012-09-02
  • Contact: Tan Xiang-ling, Professor, College of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China tanxl@ntu.edu.cn
  • About author:Zhu Li-hua, Chief physician in traditional Chinese medicine, Wuxi Hospital of Traditional Chinese Medicine, Wuxi 214001, Jiangsu Province, China 779591765@QQ.com

PDF

382

Abstract:

BACKGROUND: In vitro cell culture experiments have demonstrated that eucommia bark can induce the osteogenic differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE: To determine the active fractions of eucommia bark in osteogenic differentiation of bone marrow mesenchymal stem cells.
METHODS: Sprague-Dawley rat bone marrow mesenchymal stem cells were in vitro cultured. The active fractions of eucommia bark were extracted. The eucommia bark power was extracted by 60% ethanol, and then six ingredients were collected using high performance liquid chromatography method and numbered B2.1.1, B2.1.2, B2.1.3, B2.1.4, B2.1.5, B2.1.6. Passage 3 bone marrow mesenchymal stem cells were treated with different concentrations of eucommia bark for 6 days. At the same time, a control group was set. The expression level of alkaline phosphatase, an osteogenic differentiation marker, was determined by fluorescent quantitative PCR method.
RESULTS AND CONCLUSION: Among the six active fractions isolated by high performance liquid chromatography, B2.1.1 and B2.1.3 significantly stimulated the expression of alkaline phosphatase in bone marrow mesenchymal stem cells. The expression level of alkaline phosphatase was 3.73 and 4.74 times higher respectively compared to the control group. The active fractions for inducing osteogenic differentiation of bone marrow mesenchymal stem cells were preliminarily isolated, which provides data for finally isolating and confirming the chemical ingredients of the active fractions of eucommia bark.

CLC Number: