Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (34): 6303-6306.doi: 10.3969/j.issn.2095-4344.2012.34.007

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Effect of neurotrophic factor 3 modified Schwann cells via cationic liposome transfection on nerve defects induced by sol-gel bridging

Dong Yu-zhen1, Zong Hai-bin2, Zhao Hong-xing1, Liu Yan-xia1, Wang Ming-sheng1   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China;
    2Skill Laboratory of Basic Medical School of Xinxiang Medical College, Xinxiang 453000, Henan Province, China
  • Received:2012-01-05 Revised:2012-01-05 Online:2012-08-19 Published:2012-08-19
  • About author:Dong Yu-zhen☆, Doctor, Associate chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China dongyuzhen1998@ 163.com

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Abstract:

BACKGROUND: After peripheral nerve injury, exogenous neurotrophic factor 3 (NT-3) can promote neural regeneration and protect muscle atrophy.
OBJECTIVE: To observe the effects of NT-3 modified Schwann cells via cationic liposome transfection on repairing nerve defects induced by sol-gel bridging.
METHODS: Eighty adult Wistar rats were used to prepare left sciatic nerve defect models and then randomly divided into four groups: extracellular matrix gel group (A), Schwann cells-poly lactic acid/glycolic acid-extracellular matrix gel group (B), NT-3-polylactic acid/glycolic acid-extracellular matrix gel group (C), NT-3 modified Schwann cells-polylactic acid/glycolic acid-extracellular matrix gel group (D).
RESULTS AND CONCLUSION: ①Histological examination of gastrocnemius muscle: Gastrocnemius in group D had a clear structure based on the cross-sectional area, thick muscle fibers were thick, and presented with an essentially normal muscle structure as well as there were fewer fibrous tissues. The cross-sectional muscle area in group D was significantly greater than that in the other three groups (P < 0.05, P < 0.01). ②Myocyte apoptosis detection: The number of apoptotic cells in group D was lower than that in the other three groups (P < 0.05). These findings demonstrate that NT-3 genes modified Schwann cells by cationic liposomes transfection and extracellular matrix gel can efficiently repair nerve injury and prevent myocyte apoptosis following denervated muscle atrophy.

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