Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (34): 6284-6288.doi: 10.3969/j.issn.2095-4344.2012.34.003

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Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) nanofibers for repairing rabbit bone defects

Ye Rong1, Zhang Xiao-feng1, Yan Huai-ning1, Pan Yong-fei1, Huang Ning-ping2, Lü Lan-xin2, Jiang Zan-li3   

  1. 1Department of Orthopedics, Nanjing Armed Police Force Hospital, Nanjing 210028, Jiangsu Province, China;
    2State Key Laboratory of Bioelectronics , School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, Jiangsu Province, China;
    3Department of Orthopedics, Affiliated Zhong Da Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China  
  • Received:2011-12-12 Revised:2012-02-06 Online:2012-08-19 Published:2012-08-19
  • Contact: Zhang Xiao-feng, Master, Attending physician, Department of Orthopedics, Nanjing Armed Police Force Hospital, Nanjing 210028, Jiangsu Province, China
  • About author:Ye Rong, Attending physician, Department of Orthopedics, Nanjing Armed Police Force Hospital, Nanjing 210028, Jiangsu Province, China Yerong76@139.com

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Abstract:

BACKGROUND: The experimental studies about poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers for bone tissue regeneration are few and the research results have disputes.
OBJECTIVE: To investigate the capability of PHBV nanofibers for repairing bone defects.
METHODS: Totally 45 New Zealand white rabbits were selected. A rabbit model of bone defects in middle upper section of unilateral tibia was established. The rabbits were evenly randomized into three groups. The experimental group was implanted with PHBV nanofibers, and the control group was implanted with β-tricalcium phosphate, while nothing was implanted into the blank group. Bone formation capability, biodegradation, biocompatibility were evaluated by gross observation, radiology, histology examination and scanning electron microscope observation at corresponding time period postoperatively.
RESULTS AND CONCLUSION: At weeks 4-8 after implantation, the density of the bone defect regions was increased, osteotylus generated from osteotomy sides grew into defect region; new cortical bone was not continuous and bone defects still remained. At weeks 8-12 after implantation, new cortical bone was connected with the host cortical bone naturally, and the bone defects were perfectly healed. There was no significant difference between the experimental group and control group in new bone formation and bone defects repair at weeks 4 and 8 postoperatively (P > 0.05). At week 12 postoperatively, the capability of bone formation in the experimental group was significantly better than that in the control group, and there was a significant difference between the two groups (P < 0.05). These findings suggest that PHBV nanofibers exhibit good biocompatibility and osteoconduction, and can be used as an ideal scaffold for bone tissue engineering.

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