Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (34): 6271-6276.doi: 10.3969/j.issn.2095-4344.2012.34.001

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Osteoblastic growth peptides induce the proliferation and differentiation of rat osteoblast-like cells on titanium surface in vitro

Peng Tao1, Huang Jiao2, Xu Ling2   

  1. 1Qianjiang Central Hospital of Chongqing City, Chongqing 409000, China;
    2Chongqing Research Center for Oral Diseases and Biomedical Science, Affiliate Dental Hospital of Chongqing Medical University, Chongqing 401147, China
  • Received:2012-06-28 Revised:2012-07-12 Online:2012-08-19 Published:2012-08-19
  • Contact: Xu Ling, Associate professor, Chongqing Research Center for Oral Diseases and Biomedical Science, Affiliate Dental Hospital of Chongqing Medical University, Chongqing 401147, China xh1201@163.com
  • About author:Peng Tao, Attending physician, Qianjiang Central Hospital of Chongqing City, Chongqing 409000, China

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Abstract:

BACKGROUND: Osteogenic growth peptides have many advantages, such as low immunogenicity, self regulating sensitivity and simple extraction and making process, which can promote the proliferation activity and osteogenic activity of a variety of stromal cells.
OBJECTIVE: To observe the effect of osteogenic growth peptides on proliferation and differentiation of rat cranium derived osteoblasts on titanium surface in vitro.
METHODS: Sprague Dawley rat cranium derived osteoblasts at a concentration of 5×107/L were cultured in vitro and located on the surface of titanium disc in the 6-well plate, then the osteogenic growth peptide at concentrations of 0 (blank control), 10-10, 10-9, 10-8 and 10-7 mol/L was added. The proliferative activity was examined by MTT, and alkaline phosphatase activity was examined by enzyme-linked immunosorbent assay individually after induced for 1, 3, 5, 7 and 9 days.
RESULTS AND CONCLUSION: Compared with the blank control group, the proliferation of the osteoblasts on the surface of titanium in different osteogenic growth peptide groups was increased (P < 0.05), and the best concentration of osteogenic growth peptide was 10-9 mol/L (P < 0.05); the alkaline phosphatase activity in different osteogenic growth peptide groups was increased (P < 0.05), and the best concentration of osteogenic growth peptide was 10-8 mol/L (P < 0.05). Our results demonstrate that osteogenic growth peptides can stimulate proliferative activity and alkaline phosphatase activity of rat osteoblasts located on titanium surface in vitro.

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