Abstract:

BACKGROUND: Increased serine 307 phosphorylation of insulin receptor substrate 1 is involved in the occurrence of insulin resistance in the skeletal muscle.
OBJECTIVE: To observe the expression of insulin receptor substrate 1 and its serine 307 phosphorylation in the skeletal muscle of polycystic ovary syndrome rats.
METHODS: Female Sprague-Dawley rats were randomized to model group and control group. Insulin and human chorionic gonadotrophin were administered to the model group rats which were fed with high fat diet and 50 g/L glucose solution. The rats in the control group were given normal diet and injected normal saline daily.
RESULTS AND CONCLUSION: Compared with the control group, expression of insulin receptor substrate 1 decreased significantly (P < 0.05), while its serine 307 phosphorylation increased obviously (P < 0.05) in the model group at 6 weeks after intervention. These findings suggest that reduction of insulin receptor substrate1 protein expression and increase of its serine 307 phosphorylation are closely related with the occurrence of skeletal muscle insulin resistance in rats with polycystic ovary syndrome.