Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (33): 6102-6106.doi: 10.3969/j.issn.2095-4344.2012.33.005

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Effect of puerarin on proliferation and differentiation of osteoblasts in vitro

Wang Chang1, Tang Xu-lei2, Chen Ke-ming3, Zhang Li1   

  1. 1Affiliated Guangren Hospital. Medical School of Xi’an Jiaotong University, Xi’an No.4 Hospital, Xi’an 710004, Shaanxi Province, China;
    2Department of Endocrinology, First Affiliated Hospital of Lanzhou University, Lanzhou 730000, Gansu Province,China;
    3Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050,Gansu Province, China
  • Received:2012-01-18 Revised:2012-04-22 Online:2012-08-12 Published:2012-08-12
  • About author:Wang Chang, Master, Affiliated Guangren Hospital, Medical School of Xi’an Jiaotong University, Xi’an No.4 Hospital, Xi’an 710004, Shaanxi Province, China

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Abstract:

BACKGROUND: Osteoblasts play a key role in the process of bone metabolic balance. Phytoestrogen plays an important role in the proliferation and differentiation of osteoblasts. Puerarin acts as a kind of phytoestrogen, but the effect of puerarin with large range of concentrations on the function of osteoblasts in vitro is rarely reported.
OBJECTIVE: To investigate the effect of puerarin on the proliferation and differentiation of osteoblasts in vitro.
METHODS: The crania of Wistar rats which were born within 24 hours were obtained in aseptic condition. After enzyme digestion, isolation, purification and identification of osteoblasts, the cultured osteoblasts were randomly divided into control group and experimental groups treated with 10-3-10-10 mol/L puerarin. And then the effects of puerarin with various concentrations on proliferation of osteoblasts cultured in vitro and alkaline phosphatase activity were observed.
RESULTS AND CONCLUSION: After treated with 10-5-10-9mol/L puerarin, the proliferation of osteoblasts and the but it began to decrease at day 4; the alkaline phosphatase activity were significantly increased as compared with the control group (P < 0.05), and the proliferation of osteoblasts was fastest at day 3 (P < 0.01), but it began to decrease at day 4; the alkaline phosphatase activity between the control and experimental groups had no significant difference after inducement for 4 days (P < 0.01). However, proliferation of osteoblasts and the alkaline phosphatase activity in the 10-3 mol/L puerarin group were decreased as compared with the control group (P < 0.05). These results suggest that puerarin has a double-way effect on the in vitro osteoblasts in a dose-dependent manner. Low-dose puerarin (10-5-10-8 mol/L) can show positive functions on the forming of osteoblasts, but high-dose puerarin (10-3-10-4 mol/L) can inhibit bone formation.

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