Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (33): 6097-6101.doi: 10.3969/j.issn.2095-4344.2012.33.004

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Local expression of type I collagen fibers and changes of bone mineral density in callus tissues of diabetic rats during early fracture healing

Li Xi1, Xiang Ying-ying2, Gong Yue-kun1, Liu Jin-song1   

  1. 1Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China;
    2Stomatological School of Kunming Medical College, Kunming 650031, Yunnan Province, China
  • Received:2012-05-09 Revised:2012-05-31 Online:2012-08-12 Published:2012-08-12
  • Contact: Gong Yue-kun, Chief physician, Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China
  • About author:Li Xi, Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China lx20049@sina.com

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Abstract:

BACKGROUND: Studies have shown that local callus formation is poor in diabetic patients during fracture healing process, and the proliferation ability of osteoblasts decrease, resulting in delayed union or nonunion. Type I collagen fibers and calcium are the main components of the callus.
OBJECTIVE: To observe the local expression of type I collagen fibers and changes of bone mineral density in callus tissues of diabetic rats during early fracture healing so as to analyze the reasons for delayed union or nonunion.
METHODS: Ten rats received intraperitoneal injection of streptozotocin to induce diabetic models, and another 10 rats served as controls undergoing intraperitoneal injection of an equal volume of normal saline. Oblique fracture of the right tibia was induced in all the rats. Callus was taken for measurement at 1, 2, 4, 6 weeks after injury.
RESULTS AND CONCLUSION: In the control group, X-ray displayed callus formation, and fracture healing was significantly better than that in the model group (P < 0.01). In these two groups, the expression of type I collagen fibers showed an upward trend, reached its peak at week 2, decreased at week 4 and further declined from week 6 on. In the model group, the expression of type I collagen fibers and bone mineral density was increased but lower than those in the control group (P < 0.01). These findings indicated that the callus formation decreased during fracture healing in diabetic rats, and the increased range of type I collagen fibers and bone mineral density in the callus had a synchronous decline.

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