Abstract:

BACKGROUND: Hydrogen sulfide is a novel gaseous signal molecule, which can inhibit Ca2+ influx, open KATP channel and balance oxidation-reduction and exert other specific functions at physiological concentrations.
OBJECTIVE: To observe the effect of hydrogen sulfide on the expression of insulin-like growth factor Ⅰ, nuclear factor κB and tumor necrosis factor α in the rat hepatic ischemia-reperfusion injury model.
METHODS: Seventy SD rats were randomly divided into seven groups: sham operation, 20-minute ischemia group, 20-minute ischemia+2 and 4 hours reperfusion group, sodium hydrosulfide+20-minute ischemia group, and sodium hydrosulfide+20-minute ischemia+2 and 4 hours reperfusion group. Hepatic ischemia-reperfusion model were established by Pringle method for each group except the sham operation group. Sodium hydrosulfide+20-minute ischemia group and sodium hydrosulfide+20-minute ischemia+2 and 4 hours reperfusion group were intraperitoneally injected with 56 μmol/kg sodium hydrosulfide each day for the 5 days before surgery.
RESULTS AND CONCLUSION: The expression of insulin-like growth factor Ⅰ, nuclear factor κB and tumor necrosis factor α were significantly decreased in the ischemic rat liver tissue (P < 0.05); in comparison with the ischemia groups, both reperfusion and intraperitoneal injection of sodium hydrosulfide could significantly increase the expression of insulin-like growth factorⅠ, nuclear factor κB and tumor necrosis factor α (P < 0.05). It suggests that reperfusion after whole-liver ischemia can cause liver damage, and sodium hydrosulfide can protect the liver by increasing the expression of insulin-like growth factor Ⅰ, nuclear factor κB and tumor necrosis factor α in rat hepatic ischemia-reperfusion injury model.