Abstract:

BACKGROUND: Renal ischemia/reperfusion (I/R) induces the generation of reactive oxygen species (ROS), which leading to activation of nuclear factor-kappa B (NF-κB). NF-κB regulates production of inducible nitric oxide synthase (iNOS), resulting in increased nitric oxide production and triggering apoptosis.
OBJECTIVE: To evaluate the potential protective effect of pyrrolidine dithiocarbamate effects on NF-κB, iNOS, nitric oxide, caspase-3 and cell apoptotic index in the kidney after I/R.
METHODS: Healthy male Wistar rats were randomly divided into three groups: I/R group: underwent 45 minutes of left renal ischemia and contralateral nephrectomy, established rats renal I/R model. Pyrrolidine dithiocarbamate (PDTC) group: rats were administered PDTC (100 mg/kg intravenous bolus 30 minutes prior to I/R). Sham group: no I/R management.
RESULTS AND CONCLUSION: Compared with sham group, NF-κB, serum creatinine, urea nitrogen, iNOS, nitric oxide, caspase-3 expression and apoptosis were increased (P < 0.05). Compared with I/R group, above-mentioned indices in the PDTC group were improved. These indicate that renal I/R injury could cause renal injury and apoptosis, which was associated with NF-κB induced high expression of nitric oxide. NF-κB inhibitor PDTC obviously protects renal I/R injury.