Abstract:

BACKGROUND: The proliferation and apoptosis of hepatic stellate cells play an important role in the development of hepatocirrhosis. Octreotide that is a somatostatin derivative widely used in clinic has many biological effects, but the effect of octreotide on the proliferation and apoptosis of hepatic stellate cells is unclear.
OBJECTIVE: To investigate the effect of octreotide on the proliferation and apoptosis of rat hepatic stellate cells.
METHODS: The passage cells of hepatic stellate cells lines HSC-T6 were collected. The effect of different concentrations (1×10-², 1×10^-3, 1×10^-4, 1×10^-5, 1×10^-6, 0 mmol/L) of octreotide on the proliferation of hepatic stellate cells was detected with MTT assay after induced for 24, 48 and 72 hours. Apoptosis of hepatic stellate cells after induced by different concentration octreotide (1×10^-5, 1×10^-2 mmol/L) for 24 hours was evaluated by TUNEL staining.
RESULTS AND CONCLUSION: The higher concentration and longer induction time of octreotide, the inhibitional effect of octreotide on the proliferation of hepatic stellate cells was more obvious (in a dose- and time-dependent manner). The apoptosis of hepatic stellate cells was obviously raised with the increased concentration of octreotide. The results showed that the octreotide inhibited the proliferation of hepatic stellate cells in a dose (0-1x10^-2 mmol/L)- and time (24, 48, 72 hours)-dependent manner and could induce the apoptosis of hepatic stellate cells.