Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (31): 5793-5798.doi: 10.3969/j.issn.2095-4344.2012.31.021

Previous Articles     Next Articles

Comparison of interactions between direct and indirect cultured uterine natural killer cells and dendritic cells of mice

Wang Jing1, Wang Wei2, Weng Jing1   

  1. 1Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University Beijing 100069, China;
    2Department of Histology and Embryology, Yanjing Medical College of Capital Medical University, Beijing 100069, China  
  • Received:2012-04-06 Revised:2012-05-14 Online:2012-07-29 Published:2012-07-29
  • Contact: Weng Jing, Associate professor, Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University Beijing 100069, China wengjing@ccmu. edu.cn
  • About author:Wang Jing★, Studying for master’s degree, Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University Beijing 100069, China today1529@163.com

PDF

592

Abstract:

BACKGROUND: It has been known that uterine natural killer cells and dendritic cells have crosstalk, but the way of the interactions is controversial.
OBJECTIVE: To contrast the interactions between direct and indirect cultured uterine natural killer cells and dendritic cells of mice and then to investigate the way of their interaction.
METHODS: Dendritic cells and uterine natural killer cells were co-cultured via direct contact (direct co-culture group). Dendritic cells and uterine natural killer cells were cultured in Transwell system (indirect co-culture group). Cellular morphology was observed. The concentrations of interleukin-10, interleukin-12 and transforming growth factor-β? in supernatant were detected by enzyme-linked immunosorbent assay. The expressions of cell surface markers CD86 was detected by flow cytometry.
RSULTS AND CONCLUSION: Compared with dendritic cells and uterine natural killer cells, the concentrations of interleukin-10, interleukin-12 and transforming growth factor-the supernatant of co-culture groups were greatly increased (P < 0.05), especially the levels of the interleukin-10 (P < 0.05), the expression of CD86 of co-culture groups were also increased (P < 0.05). The concentrations of interleukin-10, interleukin-12 and transforming growth factor-β in the supernatant and the expression of CD86 of direct co-culture group were higher than those of indirect co-culture group (P < 0.05). Uterine natural killer cells can promote the maturity of dendritic cells, and dendritic cells are able to facilitate the secretion of uterine natural killer cells. The interaction way is depended on cell-cell contact.

CLC Number: