Abstract:

BACKGROUND: Fluoride may play an important role in extraperiosteal ossification of severe disabled skeletal fluorosis through inducing osteogenic differentiation of fibroblasts. Core binding factor alpha 1 (Cbfα1), as a specific transcription factor of osteoblasts, is a necessary condition for osteoblast differentiation and osteogenesis.
OBJECTIVE: To study the changes in Cbfα1 expression and proliferation activity of fibroblasts when skin fibroblasts from infant rats cultured in vitro were exposed to fluoride at different concentrations and different time.
METHODS: Supernatant of control group and seven fluoride exposed groups (0.000 1, 0.001, 0.01, 0.1, 1, 10 and 20 mg/L) were collected at 24, 48, 72 and 96 hours after fluoride, and the Cbfα1 was determined by enzyme linked immunosorbent assay. In addition, for studying the effect of fluoride on proliferation activity of fibroblast, 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide method was employed.
RESULTS AND CONCLUSION: The Cbfα1 of fibroblast was quantitatively increased by fluoride, and played an important part in the developing of extraperiosteal ossification of skeletal fluorosis. The effect of fluoride on proliferation activity of fibroblast presented in a dose-time-effect dependent manner. Shorter time and lower dose of fluoride could enhance this activity; however, as the dose increasing and time expanding, this activity was significantly decreased.