Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (28): 5222-5226.doi: 10.3969/j.issn.2095-4344.2012.28.019

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Effect of hyperuricemia on the expression of Ki-67 in rat aortic and urate anion exchanger smooth muscle cells

Song Fei1, 2, Wang Xin-mei3, Zhao Wen-juan1   

  1. 1Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China;
    2Linzi Maternity and Child Care Hospital of Shandong, Zibo 255400, Shandong Province, China;
    3Deparment of Pathology, Zibo Central Hospital, Zibo 255036, Shandong Province, China
  • Received:2011-12-03 Revised:2011-12-22 Online:2012-07-08 Published:2012-07-08
  • Contact: Zhao Wen-juan, Chief physician, Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China zhaowenjuan@medmail.com.cn
  • About author:Song Fei★, Studying for master’s degree, Attending physician, Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China; Linzi Maternity and Child Care Hospital of Shandong, Zibo 255400, Shandong Province, China wbjsf@126.com

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Abstract:

BACKGROUND: Urate anion exchanger (URAT1) plays an important role in adjusting steady-state of urate in the whole body, and ki-67 is an important cell multiplication marker. What is their effect on the aortic smooth muscle cells (ASMCs) of hyperuricemia rats?
OBJECTIVE: To observe the effect of hyperuricemia on the expression of Ki-67 in ASMCs and URAT1.
METHODS: Twenty 8-week-old Wistar rats were randomly divided into two groups: control group (n=10) and experimental group (n=10). Rats in the experimental group were fed with high yeast and gavaged with amidopurine fluid. However, rats in the control group were fed with general diet and gavaged with the same dose of distilled water. When the level of blood uric acid started to show the difference, all the rats were fed for another 5 weeks and then were sacrificed. Hematoxylin-eosin staining was used to observe the structure of aortic smooth muscle and immunohistochemistry was used to detect the expression of Ki-67 in ASMCs and URAT1.
RESULTS AND CONCLUSION: Hyperuricemia that persisted for 5 weeks had no obvious effect on the expression of Ki-67 and in ASMCs and URAT1 in 16-week-old male Wistar rats, or did not result in atherosclerosis. As an influential factor of atherosclerosis, hyperuricemia needs more time to result in atherosclerosis.

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