Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (28): 5206-5211.doi: 10.3969/j.issn.2095-4344.2012.28.016

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Proteomics research on the joint synovium of patients with rheumatoid arthritis

Chen Jiang1, Xiong Xin-gui2, Liang Qing-hua2, Yang Bo2, Wu Dan2   

  1. 1Center of Telemedicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China;
    2Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
  • Received:2011-12-02 Revised:2011-12-26 Online:2012-07-08 Published:2012-07-08
  • Contact: Xiong Xin-gui, Doctor, Attending physician, Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China xxg_xyz@ 126.com Liang Qing-hua, Professor, Doctoral supervisor, Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China lqhxy@126.com
  • About author:Chen Jiang★, Master, Engineer, Center of Telemedicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China

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Abstract:

BACKGROUND: Several studies have shown that it can result in joint cartilages and bones breaking due to the sustained attack of synovitis. But reports about proteomics research on the joint synovium of rheumatoid arthritis (RA) patients in China are few.
OBJECTIVE: To explore the possible underlying mechanism of RA and to seek out RA associated proteins by comparing protein expression difference between the synovium of patients with surgical trauma and patients with RA.
METHODS: The synovial tissues of six patients with surgical trauma and six patients with RA were obtained by arthroscopic surgery and they were divided into RA group and control group. The total proteins were extracted from synovial tissue and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE), which was followed by Coomassie Brilliant Blue staining to visualize the protein spots in the 2-DE. The differentially expressed proteins were analyzed by using PDQUEST analysis software, and then identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and homogonously compared and identified in Swiss Prot and NCBInr databases with Mascot software.
RESULTS AND CONCLUSION: The 2-DE patterns of RA group and control group were established. Proteins were separated by the 2-DE, and 130 proteins were found differentially expressed over two folds between synovium of surgical trauma and RA patients. Thirty-nine clear proteins of them were selected and analyzed with MALDI-TOF-MS and 29 proteins were identified. Among the identified differential expression proteins, 21 proteins were up-regulated and eight were down-regulated, involved in substance metabolism, signaling pathway, anti-oxidation protection and molecule chaperone. These findings suggest that synovium lesions of RA are a complex process in which multiple proteins are involved, and some differentially expressed proteins may play a potential role in the pathogenesis of RA.

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