Abstract:

BACKGROUND: In the early stage of hypoxia-reoxygenation, the measures of promoting proliferation myocardium microvascular endothelial cells (MMECs) involve a series of related genetic changes, which are regulated by multiple genes.
OBJECTIVE: To investigate the effects of diazoxide pretreatment on the mRNA expression of PI3K, Akt and FKN as well as proliferation of rat MMECs exposed to hypoxia-reoxygenation.
METHODS: The SD rat MMECs were randomly divided into normal control group, hypoxia-reoxygenation group, diazoxide pretreatment group and diazoxide pretreatment+5-hydroxydecanoate group. Cell vitality, morphology, apoptotic rate and mRNA expression of PI3K, Akt and FKN were detected.
RESULTS AND CONCLUSION: Compared with the normal control group, the cell proliferation rate was significantly decreased and the mRNA expression of FKN and Akt was up-regulated in the hypoxia-reoxygenation group (P < 0.01). Compared with the hypoxia-reoxygenation group, the cell proliferation rate was significantly increased (P < 0.05), and the mRNA expression of PI3K and Akt was up-regulated obviously, while FKN mRNA expression was significantly decreased in diazoxide pretreatment group (P < 0.01). Diazoxide pretreatment+5-hydroxydecanoate group called off diazoxide pretreatment-induced changes, and did not differ from the hypoxia-reoxygenation group. These findings suggest that diazoxide pretreatment can promote cell proliferation and up-regulate the mRNA expression of PI3K and Akt as well as decrease FKN mRNA expression protect rat MMECs from hypoxia-reoxygenation.