Abstract:

BACKGROUND: Today’s hot issue in the tissue engineering is to strengthen or reconstruct the function of seeding cells by gene transfection technology. But osteoblasts transfected by platelet-derived growth factor A (PDGF-A) for periodontal tissue regeneration is rarely reported.
OBJECTIVE: To observe the influence of PDGF-A gene transfection on osteoblasts growth.
METHODS: The eukaryotic expression plasmid of PDGF-A was constructed. 3T3 cells were isolated and transfected with various methods. The gene expression of PDGF-A in osteoblasts was detected by reverse transcription-PCR method, and cells proliferation activity was detected with MTT method, as well as the migration of cells was observed by the experiment of cells scratch test in vitro.
RESULTS AND CONCLUSION: The results of reverse transcription-PCR showed that the expression of osteoblasts transfected by eukaryotic expression plasmid carrying PDGF-A was significantly increased, and growth rate and migration speed was accelerated obviously (P < 0.05). These findings suggest that the limposome mediator PDGF-A gene can transfect osteoblasts successfully to promote the growth, proliferation and migration of osteoblasts, laying a foundation for PDGF-A gene treatment in periodontal tissue regeneration.