Effects of Chinese medicine for detoxifying and removing blood stasis in model mice with acute lymphocytic leukemia

doi:10.3969/j.issn.2095-4344.1391

Abstract

Abstract:

BACKGROUND: Studies have shown that early chemotherapy is essential for delaying the progression of leukemia. Chinese herbal medicine has little effect on leukemia, but some studies hold the opposite opinion that Chinese herbal medicine combined with chemotherapy can improve the cure rate of patients to a certain extent. Due to the complex composition, the therapeutic mechanism of Chinese herbal medicines is still unclear.
OBJECTIVE: To explore the effects of Chinese medicine for detoxifying and removing blood stasis in mice with acute lymphoblastic leukemia.
METHODS: Leukemia cell line L1210 and leukemia drug-resistant cells were injected into mice via the tail vein to prepare mouse models of acute lymphoblastic leukemia. These models were then divided into leukemia model group, low- and high-concentration Chinese medicine for detoxifying and removing blood stasis groups, and normal group was set for controls. At the second day after modeling, Chinese medicine for detoxifying and removing blood stasis (10 and 50 mg/kg) was given for 7 days in the low- and high-concentration treatment group, respectively. Blood samples from the tail vein were taken at 7:00 a.m. on Monday, for 5 consecutive weeks. MTT was used to detect the survival rate of leukemia drug-resistant cells, and blood cell types and hemogram indexes were analyzed. The expression levels of NF-κB/P65, NF-κB/P50, IκBα, phosphatase and tensin homolog deleted on chromosome ten (PTEN), Akt, Bcl-2 and p-Akt were detected by western blot assay.
RESULTS AND CONCLUSION: (1) Compared with the leukemia model group, the survival rate of leukemia drug-resistant cells was significantly decreased in the low- and high-concentration treatment groups. (2) Compared with the leukemia model group, the expression levels of NF-κB/P65, NF-κB/P50, IκBα, Bcl-2, Akt and p-Akt were significantly decreased in the low- and high-concentration treatment groups, while the expression level of PTEN increased significantly (P < 0.01). (3) Compared with the leukemia model group, the leukocytes, the number of white blood cells, lymphocytes and L1210 cells in the low- and high-concentration treatment groups was significantly decreased, and the number of neutrophils was significantly increased (P < 0.01). (4) To conclude, Chinese medicine for detoxifying and removing blood stasis can inhibit the proliferation of L1210 cells by blocking the PI3K/Akt and NF-κB signaling pathway to alleviate and treat acute lymphoblastic leukemia.

Key words: leukemia, Chinese medicine for detoxifying and removing blood stasis, leukemia cell line, leukemia drug-resistant cells, Chinese herbal adjuvant therapy, PI3K/Akt signaling pathway

CLC Number:

Dang Hui1, Zhang Shuxiang1, Guan Xutao1, Wan Jiangwei1, Shi Lin2. Effects of Chinese medicine for detoxifying and removing blood stasis in model mice with acute lymphocytic leukemia[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(27): 4392-4396.

Figures/Tables 5

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